{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:30:38Z","timestamp":1775521838394,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2004,9,10]],"date-time":"2004-09-10T00:00:00Z","timestamp":1094774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Oral submucous fibrosis (OSF) is associated with the betel quid chewing habit, and 86% of betel quid chewers in Taiwan are also smokers. Arecoline and safrole are major principles in the composition of betel quid, and nicotine is the main toxic ingredient of cigarettes.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> To explore the pathogenesis of OSF, flow cytometry was used to compare collagen phagocytosis by fibroblasts from the normal and the OSF region of the same 15 OSF patients.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The results indicated that heterogeneity of fibroblasts existed because collagen phagocytosis by fibroblasts from the normal region was higher than from the OSF region in the same patient. The percentage of phagocytic cells was significantly inhibited by 10, 25 and 50\u2003<jats:italic>\u03bc<\/jats:italic>g\/ml arecoline, safrole and nicotine in normal fibroblast cultures, respectively, and the percentage of phagocytic cells was significantly reduced by 25, 25 and 50\u2003<jats:italic>\u03bc<\/jats:italic>g\/ml arecoline, safrole and nicotine in OSF fibroblast cultures, respectively. Collagen phagocytosis by fibroblasts exhibited prominent dose\u2010dependent inhibition as the concentration of arecoline, safrole, and nicotine increased. Besides, nicotine had a synergistic effect on arecoline\u2010 or safrole\u2010inhibited collagen phagocytosis.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The present study concludes that even in the same person, the collagen phagocytosis by fibroblasts is different between normal and OSF region. The deficiency in collagen phagocytosis by fibroblasts of the lesion might participate in the pathogenesis of OSF. Arecoline, safrole and nicotine, which are released in saliva during BQ chewing plus cigarette smoking, inhibit collagen phagocytosis by fibroblasts in a dose\u2010dependent manner and may induce OSF formation in Taiwan's patients.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2004.00229.x","type":"journal-article","created":{"date-parts":[[2004,9,10]],"date-time":"2004-09-10T08:45:33Z","timestamp":1094805933000},"page":"581-587","source":"Crossref","is-referenced-by-count":36,"title":["Effects of arecoline, safrole, and nicotine on collagen phagocytosis by human buccal mucosal fibroblasts as a possible mechanism for oral submucous fibrosis in 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