{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T02:08:08Z","timestamp":1780538888147,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,10]],"date-time":"2005-01-10T00:00:00Z","timestamp":1105315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Keratinocyte growth factor\u20101 (KGF\u20101) is the seventh member of the fibroblast growth factor family. KGF\u20101 is produced by mesenchymal cells such as fibroblasts and upregulated in a variety of hyperplastic tissues. Currently, there is limited information about the regulation of KGF\u20101 expression in areca quid\u2010associated oral submucous fibrosis (OSF). The aim of the study was to compare KGF\u20101 expression in normal human buccal mucosa and OSF specimens and further to explore the potential mechanism that may lead to induce KGF\u20101 expression.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The expression of KGF\u20101 from fibroblasts cultured from OSF and normal buccal mucosa were using reverse\u2010transcriptase polymerase chain reaction and enzyme\u2010linked immunosorbent assay. In addition, arecoline, a major areca nut alkaloid, was challenged to normal buccal mucosa fibroblasts (BMFs) to elucidate whether KGF\u20101 expression could affect by arecoline. Furthermore, 25 OSF specimens and six normal buccal mucosa specimens were examined by immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Fibroblasts derived from OSF were found to exhibit higher KGF\u20101 expression than BMFs both in mRNA and protein levels (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). In addition, upregulation of KGF\u20101 mRNA gene and protein expression were found in BMFs stimulated by arecoline (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). From the results of immunohistochemistry, KGF\u20101 expression was significantly higher in OSF specimens and expressed mainly by fibroblasts, endothelial cells, inflammatory cells, and epithelial cells.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Taken together, these results suggest that KGF\u20101 expression is significantly upregulated in OSF tissues from areca quid chewers and arecoline may be responsible for the enhanced KGF\u20101 expression <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2004.00288.x","type":"journal-article","created":{"date-parts":[[2005,1,10]],"date-time":"2005-01-10T10:03:10Z","timestamp":1105351390000},"page":"100-105","source":"Crossref","is-referenced-by-count":25,"title":["Raised keratinocyte growth factor\u20101 expression in oral submucous fibrosis <i>in vivo<\/i> and upregulated by arecoline in human buccal mucosal fibroblasts <i>in vitro<\/i>"],"prefix":"10.1111","volume":"34","author":[{"given":"Chung\u2010Hung","family":"Tsai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun\u2010Fa","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Juai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Yung","family":"Chou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Chao","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,10]]},"reference":[{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/13556210120091464"},{"key":"e_1_2_11_3_2","first-page":"141","volume-title":"Betel\u2010quid and Areca\u2010nut Chewing. 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