{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T01:45:14Z","timestamp":1784684714574,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,6,6]],"date-time":"2005-06-06T00:00:00Z","timestamp":1118016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Synovitis, which is characterized by infiltration of inflammatory cells, often accompanies progression of clinical symptoms of the temporomandibular joint (TMJ). Synovial fibroblasts of the TMJ are believed to play important roles in progression of synovitis. The purpose of this study was to examine production and gene expression of chemokines by synovial fibroblasts stimulated by tumor necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic> (TNF\u2010<jats:italic>\u03b1<\/jats:italic>).<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Protein levels of chemokines were measured by enzyme\u2010linked immunosorbent assay (ELISA). Gene expression of chemokines was analyzed by real\u2010time polymerase chain reaction (PCR).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Production of interleukin (IL)\u20108, growth\u2010related oncogene (GRO)\u2010<jats:italic>\u03b1<\/jats:italic>, monocyte chemoattractant protein (MCP)\u20101, and regulated upon activation normal T\u2010cell expressed and secreted (RANTES) protein by synovial fibroblasts was increased by TNF\u2010<jats:italic>\u03b1<\/jats:italic>. In contrast, stromal cell\u2010derived factor (SDF)\u20101<jats:italic>\u03b1<\/jats:italic>, macrophage inflammatory protein (MIP)\u20101<jats:italic>\u03b1<\/jats:italic> and \u20101<jats:italic>\u03b2<\/jats:italic> were not detectable in conditioned media of synovial fibroblasts, with or without TNF\u2010<jats:italic>\u03b1<\/jats:italic> treatment. Increases in gene expression of IL\u20108, GRO\u2010<jats:italic>\u03b1<\/jats:italic>, MCP\u20101, and RANTES in response to TNF\u2010<jats:italic>\u03b1<\/jats:italic> treatment were detected.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Increased protein production and gene expression of chemokines by synovial fibroblasts in response to TNF\u2010<jats:italic>\u03b1<\/jats:italic> treatment appears to play an important role in recruitment of inflammatory cells into synovium and the progression of synovitis in the TMJ.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2005.00302.x","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T10:45:57Z","timestamp":1126521957000},"page":"357-363","source":"Crossref","is-referenced-by-count":39,"title":["Tumor necrosis factor\u2010<i>\u03b1<\/i> increases chemokine gene expression and production in synovial fibroblasts from human temporomandibular joint"],"prefix":"10.1111","volume":"34","author":[{"given":"Naomi","family":"Ogura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makiko","family":"Tobe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sakamaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Nagura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimitsu","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshirou","family":"Kondoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,6]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(97)90246-8"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-5182(05)80452-4"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0901-5027(97)80838-7"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(98)90609-7"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1054\/ijom.2002.0357"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780331204"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(94)90006-X"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/36.4.420"},{"key":"e_1_2_5_10_2","first-page":"285","article-title":"Activated gelatinase\u2010B (MMP\u20109) and urokinase\u2010type plasminogen activator in synovial fluids of patients with arthritis. 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