{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T18:41:47Z","timestamp":1767984107159,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,8,7]],"date-time":"2006-08-07T00:00:00Z","timestamp":1154908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> 1<jats:italic>\u03b1<\/jats:italic>,25\u2010Dihydroxyvitamin D<jats:sub>3<\/jats:sub> [1,25(OH)<jats:sub>2<\/jats:sub> Vitamin D<jats:sub>3<\/jats:sub>] induces growth inhibition in squamous cell carcinoma (SCC) cell lines of the head and neck by arresting the cells in the G0\/G1 phase of the cell cycle, probably due to an enhanced expression of p21, which could be demonstrated in other cell lines (JPPA, SCC9) before. In SCC25, a SCC cell line isolated from tongue, growth inhibition but no overexpression of p21 was detected. The retinoblastoma gene, as a direct target of G1 cyclin\u2013CDK complexes, showed an obvious shift from the hyperphosphorylated to the hypophosphorylated form under 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub>, which indicates that the growth inhibition takes place in the G0\/G1 phase. To explore the possible pathway of growth inhibition in SCC25 we investigated other cell cycle inhibitors (p18, p19, p27).<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Synchronized cells were treated with 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub> over 96\u2003h. The cell cycle status and expression of cell cycle\u2010regulating proteins was determined by fluorescence\u2010activated cell sorting (FACS) and Western blotting.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> An overexpression of p18 in 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub> vs. ethanol\u2010treated cells was determined until 30\u2003h in SCC25. No influence was detectable on the expression of p27 and p19.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> One mechanism by which 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub> controls cell growth might be the upregulation of p21. As p21 was unsusceptible to 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub> in SCC25, other inhibiting proteins were necessary to be tested. The proven upregulation of p18 seems to be the responsible step for growth inhibition of 1,25(OH)<jats:sub>2<\/jats:sub>Vitamin D<jats:sub>3<\/jats:sub> in SCC25.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2006.00407.x","type":"journal-article","created":{"date-parts":[[2006,8,7]],"date-time":"2006-08-07T09:40:51Z","timestamp":1154943651000},"page":"472-478","source":"Crossref","is-referenced-by-count":23,"title":["1,25(OH)<sub>2<\/sub>Vitamin D<sub>3<\/sub> induces elevated expression of the cell cycle inhibitor p18 in a squamous cell carcinoma cell line of the head and neck"],"prefix":"10.1111","volume":"35","author":[{"given":"Claudia","family":"Gedlicka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudrun","family":"Hager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Weissenb\u00f6ck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelm","family":"Gedlicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Knerer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Kornfehl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Formanek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-0709(97)90022-3"},{"key":"e_1_2_5_3_2","first-page":"805","article-title":"Antiproliferative effects of 1,25\u2010dihydroxyvitamin D3 on primary cultures of human prostatic cells","volume":"54","author":"Peehl DM","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00178289"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/000164801300006353"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90543-6"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/scel.1994.1047"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90499-G"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01753887"},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"7511","DOI":"10.1016\/S0021-9258(18)42547-1","article-title":"Identification of lamin D and histones as 1,25\u2010dihydroxyvitamin D3\u2010regulated nuclear phosphoproteins in HL\u201060 cells","volume":"267","author":"Martell RE","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/15419069809040285"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401043"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-0026"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.10.745"},{"key":"e_1_2_5_15_2","first-page":"3355","article-title":"Effects of 1,25\u2010(OH)2 D3, EB1089 and analog V on PTHrP production, PTHrP mRNA expression and cell growth in SCC2\/88","volume":"21","author":"Kunakornsawat S","year":"2001","journal-title":"Anticancer Res"},{"key":"e_1_2_5_16_2","first-page":"56","article-title":"Targeted disruption of the 25\u2010hydroxyvitamin D3 1\u03b1\u2010hydroxylase gene in ras\u2010transformed keratinocytes demonstrates that locally produced 1\u03b1,25\u2010dihydroxyvitamin D3 suppresses growth and induces differentiation in an autocrine fashion","volume":"1","author":"Huang DC","year":"2002","journal-title":"Mol Cancer Res"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2005.06.001"},{"key":"e_1_2_5_18_2","doi-asserted-by":"crossref","first-page":"3472S","DOI":"10.1093\/jn\/134.12.3472S","article-title":"Vitamin\u00a0D and skin cancer","volume":"134","author":"Bikkle DD","year":"2004","journal-title":"J Nutr"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.143.7.2508"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0385-8146(03)00091-9"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/00016480310015353"}],"container-title":["Journal of Oral Pathology &amp; 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