{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:17:41Z","timestamp":1770337061635,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,8,7]],"date-time":"2006-08-07T00:00:00Z","timestamp":1154908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Radicular cysts are the most common cysts in human jaw bones. These lesions induce bone remodeling of the surrounding alveolar bones, which was termed \u2018condensing osteitis\u2019, and was suggested to be related to cells of the osteoblastic lineage. The Runx2 (core\u2010binding protein [cbfa]1\/polyoma enhancer\u2010binding protein [pebp]2<jats:italic>\u03b1<\/jats:italic>A) was shown to be a DNA\u2010binding transcriptional molecule expressed in osteoprogenitor cells.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We confirmed the specificity of anti\u2010Runx2 antiserum, using Western blotting analysis. We investigated the expression and localization of Runx2 in 32 radicular cyst cases with bone tissue fragments, immunohistochemically.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Signals for Runx2 were seen in 18 cases (56.3%) of radicular cysts with bone formation. These signals were immunolocalized in the nuclei of the spindle\u2010shaped osteoprogenitor cells in the cyst walls, whereas only a few signals were seen in the cuboidal osteoblastic cells near the fibrous bones. Signals for type I collagen were immunolocalized in the dense collagen fibers in the cyst walls and in the matrix of the fibrous bone around the radicular cysts, whereas no signals were seen on the inner portions with inflammatory cell infiltration of the cyst walls. Very weak signals for transforming growth factor (TGF)\u2010<jats:italic>\u03b2<\/jats:italic>1 were infrequently seen in the osteoblasts of the fibrous bone, whereas signals for TGF\u2010<jats:italic>\u03b2<\/jats:italic>2 were observed in young osteocytes in the fibrous bones, in B\u2010cell lymphocytes infiltrating into the inner portions, and on the cellular membranes of the lining epithelium.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The nuclear expression of Runx2 in spindle\u2010shaped cells in the outer portions may play an essential role in the induction of fibrous bone tissue around radicular cysts. TGF\u2010<jats:italic>\u03b2<\/jats:italic>2 may play a role in the production of type I collagen, which acts as a template for pathologic new bone formation, in radicular cysts.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2006.00426.x","type":"journal-article","created":{"date-parts":[[2006,8,7]],"date-time":"2006-08-07T09:40:51Z","timestamp":1154943651000},"page":"492-499","source":"Crossref","is-referenced-by-count":6,"title":["Runx2 expression is associated with pathologic new bone formation around radicular cysts: an immunohistochemical demonstration"],"prefix":"10.1111","volume":"35","author":[{"given":"Kimihide","family":"Kusafuka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Sasaguri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadao","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamiko","family":"Takemura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Kameya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,7]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"493","volume-title":"A textbook of oral pathology, 4th edn. 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