{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:34:56Z","timestamp":1770824096205,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T00:00:00Z","timestamp":1166572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Although human papillomavirus (HPV) infection has been shown to be a significant carcinogen in cervical squamous cell carcinoma (SCC), its significance in oral SCC remains unclear.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We developed highly sensitive detection methods for HPV to elucidate the prevalence and localization of HPV in paraffin sections from human oral SCC using modified <jats:italic>in situ<\/jats:italic> polymerase chain reaction (PCR) and <jats:italic>in situ<\/jats:italic> hybridization AT tailing (ISH\u2010AT). Analyses revealed a high prevalence of several HPV types (HPV\u201016, \u201018, \u201022, \u201038 and \u201070) under optimal conditions. The ISH\u2010AT method can be used as an alternative to <jats:italic>in situ<\/jats:italic> PCR.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Various staining patterns were observed in the 20 cases examined, and HPV\u2010positive cells were localized within the surface epithelium as well as in neoplastic cells. We demonstrated that HPV\u2010DNA could be detected in paraffin sections using either the method of <jats:italic>in situ<\/jats:italic> PCR or ISH, providing an appropriate primer and probe are used.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results suggest that HPV infection could be one of several risk factors being involved in oral SCC.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2006.00490.x","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T13:02:24Z","timestamp":1282136544000},"page":"18-24","source":"Crossref","is-referenced-by-count":27,"title":["Highly sensitive detection of HPV\u2010DNA in paraffin sections of human oral carcinomas"],"prefix":"10.1111","volume":"36","author":[{"given":"Koichi","family":"Koyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Uobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,20]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0738-081X(96)00164-2"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2003.12.011"},{"key":"e_1_2_5_4_2","first-page":"F55","article-title":"Papillomavirus infections\u2013a major cause of human cancers","volume":"1288","author":"Zur Hausen H","year":"1996","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19960601)77:11<2275::AID-CNCR14>3.0.CO;2-U"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880113"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910610403"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1054\/ijom.2002.0443"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(00)00075-0"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2001.115392"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjom.2000.0162"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/339193"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00098-7"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(97)00081-X"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(98)00028-1"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/154411130401500402"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.1999.140401.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1991.tb00936.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63833-3"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00072-5"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2002.00129.x"},{"key":"e_1_2_5_22_2","first-page":"243","article-title":"Examination and evaluation of the usefulness of its in situ PCR detection technique for human papillomavirus in squamous cell carcinoma","volume":"64","author":"Tsujimoto K","year":"2001","journal-title":"J Osaka Odontol Soc"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-60592-5_3"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00112-9"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.5843\/jsot.12.307"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-7-1825"},{"key":"e_1_2_5_27_2","first-page":"4171","article-title":"Detection and quantitation of human papillomavirus (HPV) DNA in the sera of patients with HPV\u2010associated head and neck squamous cell carcinoma","volume":"6","author":"Capone RB","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(96)80378-7"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.3353\/omp.4.17"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.1.87"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0964-1955(94)90036-1"}],"container-title":["Journal of Oral Pathology &amp; 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