{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:29:23Z","timestamp":1770751763092,"version":"3.50.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,1,23]],"date-time":"2008-01-23T00:00:00Z","timestamp":1201046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Heat shock protein (HSP) 47, a collagen\u2010specific molecular chaperone, is involved in the processing and\/or secretion of procollagen. HSP47 is consistently and dramatically upregulated in a variety of fibrotic diseases. The aim of this study was to compare HSP47 expression in normal human buccal mucosa and oral submucous fibrosis (OSF) specimens and further to explore the potential mechanisms that may lead to induce HSP47 expression.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The mRNA levels of HSP47 from fibroblasts cultured from 20 OSF and 10 normal buccal mucosal fibroblasts (BMFs) were evaluated by reverse transcription polymerase chain reaction. The effect of arecoline, the major areca nut alkaloid, was added to explore the potential mechanisms that may lead to induce HSP47 expression. Furthermore, mitogen\u2010activated protein kinase kinase (MEK) inhibitor U0126, phosphatidylinositol 3\u2010kinase (PI3K) inhibitor LY294002, p38 inhibitor SB203580, cyclo\u2010oxygenase\u20102 (COX\u20102) inhibitor NS\u2010398, and glutathione precursor <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine were added to find the possible mechanisms.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> OSF demonstrated significantly higher HSP47 mRNA expression than BMFs (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). Arecoline was also found to elevate HSP47 mRNA expression in a dose\u2010dependent manner (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). The amount of HSP47 was about 3.7\u2010fold at a concentration level of 80\u2003\u03bcg\/ml arecoline when compared with control (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). In addition, pre\u2010treatment with pharmacologic agents markedly inhibited the arecoline\u2010induced HSP47 mRNA expression (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Taken together, HSP47 is significantly upregulated in OSF from areca quid chewers and HSP47 expression induced by arecoline in fibroblasts may be mediated by MEK, PI3K, and COX\u20102 signal transduction pathways.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2007.00633.x","type":"journal-article","created":{"date-parts":[[2010,8,12]],"date-time":"2010-08-12T11:37:52Z","timestamp":1281613072000},"page":"206-210","source":"Crossref","is-referenced-by-count":24,"title":["The upregulation of heat shock protein 47 expression in human buccal fibroblasts stimulated with arecoline"],"prefix":"10.1111","volume":"37","author":[{"given":"Shun\u2010Fa","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung\u2010Hung","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Chao","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,23]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"224","article-title":"Oral submucous fibrosis as a precancerous condition","volume":"92","author":"Pindorg JJ","year":"1984","journal-title":"Scand J Dent Res"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1990.tb00804.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1994.tb00258.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4805876"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1990.tb00821.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(86)90112-3"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb02096.x"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00045-8"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00083-5"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2003.00004.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00288.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.05.006"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(02)00123-9"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01272.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.06.007"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.08.009"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01172-9"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2006.12.001"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47412-1"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4257"},{"key":"e_1_2_5_22_2","first-page":"1183","article-title":"Immunolocalization of collagen and collagen\u2010binding heat shock protein 47 in fibrotic lung diseases","volume":"11","author":"Razzaque MS","year":"1998","journal-title":"Mod Pathol"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050191"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(00)00121-4"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1992.tb01007.x"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2005.08.004"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007840050074"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(00)00262-9"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007840000085"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.296.5573.1655"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.08.003"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404465200"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21104"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-8-37"}],"container-title":["Journal of Oral Pathology &amp; 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