{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:31:12Z","timestamp":1759422672329},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,12,30]],"date-time":"2008-12-30T00:00:00Z","timestamp":1230595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> In this study, we analyzed the gene expression profile of fibroblast\u2010like synoviocyte (FLS) cultures from the temporomandibular joint (TMJ) to identify candidate genes associated with intracapsular pathologic conditions of TMJ. Cyclooxygenase (COX)\u20102 was one of the genes in FLS upregulated following stimulation by interleukin (IL)\u20101\u03b2, a cytokine thought to play a key role in several pathological conditions. This study investigated the expression of COX\u20101 and COX\u20102 in cultured human FLS and rat TMJ synovium following stimulation with IL\u20101\u03b2.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> RNA was isolated from human FLS after IL\u20101\u03b2 treatment. COX\u20101 and \u20102 expression was examined using a GeneChip and real\u2010time polymerase chain reaction. Prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) levels in conditioned media from FLS were measured using enzyme\u2010linked immunosorbent assay. Synovial tissues from TMJs of IL\u20101\u03b2\u2010injected rats were examined for COX\u20101 and COX\u20102 expression by immunohistochemical staining.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Following treatment of FLS with IL\u20101\u03b2, expression of the COX\u20102 gene increased up to 8\u2003h and peaked at 4\u2003h, whereas COX\u20101 expression did not change. Stimulation with IL\u20101\u03b2 increased the level of PGE<jats:sub>2<\/jats:sub> in conditioned media of cultured FLS in a time\u2010dependent manner up to 48\u2003h. Immunohistochemistry showed a strong positive staining for COX\u20102 in the lining and sub\u2010lining synovial tissues of the TMJ of IL\u20101\u03b2\u2010injected rats. In contrast, staining for COX\u20101 was the same in synovial tissues with and without IL\u20101\u03b2 injection.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These data suggest that COX\u20102 expression stimulated by IL\u20101\u03b2 stimulates the production of PGE<jats:sub>2<\/jats:sub> in FLS and plays important roles in the progression of inflammation in TMJ.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2008.00733.x","type":"journal-article","created":{"date-parts":[[2009,7,10]],"date-time":"2009-07-10T11:03:35Z","timestamp":1247223815000},"page":"584-590","source":"Crossref","is-referenced-by-count":16,"title":["Expression of cyclooxygenase\u20101 and \u20102 in IL\u20101\u03b2\u2010induced synovitis of the temporomandibular joint"],"prefix":"10.1111","volume":"38","author":[{"given":"K.","family":"Satoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ogura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Akutsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Kuboyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kuyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Kondoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-5182(05)80452-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(97)90246-8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0901-5027(97)80838-7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(98)90609-7"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1054\/ijom.2002.0357"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780331204"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(94)90006-X"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.3.569"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.139.6.1902"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar331"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00259-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02274836"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(98)90256-7"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(98)90415-2"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2007.00515.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.02.040184.002003"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1385\/IR:22:2-3:319"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35068566"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117060"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71504-4"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2004.01.026"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1053\/joms.2000.16619"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2002.310606.x"},{"key":"e_1_2_6_25_2","article-title":"Inhibition of cyclooxygenase 2 expression by diallyl sulfide on joint inflammation induced by urate crystal and IL\u20101\u03b2","author":"Lee H\u2010S","year":"2008","journal-title":"Osteoarthritis Cartilage"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2005.00302.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11261"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.5.2790"},{"key":"e_1_2_6_29_2","first-page":"1747","article-title":"The role of hypoxia on expression of cyclooxygenase\u20102 in synovial fibroblasts derived from human temporomandibular joint","volume":"85","author":"Ke J","year":"2005","journal-title":"Zhonghua Yi Xue Za Zhi"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600412"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2842.2002.00969.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.41-3-235"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(00)90335-5"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2005.10.068"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2003.09.016"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600516"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0714.2008.00733.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0714.2008.00733.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:52:52Z","timestamp":1697071972000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0714.2008.00733.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1111\/j.1600-0714.2008.00733.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0714.2008.00733.x","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]}}}