{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:33:47Z","timestamp":1791614027007,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,12,17]],"date-time":"2010-12-17T00:00:00Z","timestamp":1292544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p> <jats:italic>J Oral Pathol Med<\/jats:italic> (2010) <jats:bold>40<\/jats:bold>: 369\u2013379<\/jats:p><jats:p><jats:bold>Purpose:\u2002<\/jats:bold> To explore the presence of differentially expressed proteins in OSCC for discrimination of tumour and normal mucosa to establish potential biomarkers and therapeutic targets.<\/jats:p><jats:p><jats:bold>Experimental Design:\u2002<\/jats:bold> Paired protein samples of 12 individuals (tongue cancer and non\u2010cancerous mucosa) were separated by two\u2010dimensional polyacrylamid gel electrophoresis. The protein patterns were compared pairwise and protein spots were quantified. We identified about 70 regulated proteins which we subsequently identified by MALDI\u2010TOF mass spectrometry.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Cancerous and non\u2010cancerous tissues could be most precisely distinguished by a panel of proteins. They include the heat shock proteins (hsp)70 and 90, keratins (ck) 5, 6, 13, 14, 16, 17 and 19, beta globin, alpha\u20102\u2010actin, stratifin, tropomyosin, calreticulin precursor, beta\u20102\u2010tubulin, galectin7, thioredoxin, involucrin, adenylyl\u2010cyclase\u2010associated protein, disulfide isomerase\u2010associated protein, thyrosine 3\u2010monooxygenase, MYL2 and the s100 calcium binding protein. MYL3, cardiac muscle alpha actin 1 proprotein and transferrin were under\u2010represented in OSCC. Six biomarkers, ck6 und ck13, beta globin, alpha\u20102\u2010actin, hsp70 and hsp90 discriminated best between cancerous and non\u2010cancerous oral tissues. All over\u2010expressed proteins were analysed by STRING\u2010analysis to highlight experimentally determined and computationally predicted interactions between the proteins. Especially involucrin, hsp70, calreticulin precursor, stratifin, (ck) 5, 6, 14, 19, tyrosine 3\u2010monooxygenase, beta\u20102\u2010tubulin and disulfide isomerase associated protein showed multiple relations.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> We identified six proteins which are differentially expressed in most OSCC compared to healthy tissues. Of those, by string analysis, multiple interaction partners are assumed for hsp70. This protein is supposed to be the most promising candidate as marker molecule and target for OSCC therapy.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2010.00982.x","type":"journal-article","created":{"date-parts":[[2010,12,17]],"date-time":"2010-12-17T12:06:24Z","timestamp":1292587584000},"page":"369-379","source":"Crossref","is-referenced-by-count":36,"title":["Analysis of differentially expressed proteins in oral squamous cell carcinoma by MALDI\u2010TOF MS"],"prefix":"10.1111","volume":"40","author":[{"given":"Uta J. 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