{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:28:14Z","timestamp":1789727294164,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T00:00:00Z","timestamp":1311811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p><jats:italic>J Oral Pathol Med<\/jats:italic> (2012) <jats:bold>41<\/jats:bold>: 141\u2013148<\/jats:p><jats:p><jats:bold>Background:\u2002<\/jats:bold> Multiple drug resistance protein 1 (MRP1), lung resistance protein (LRP), topoisomerase II\u03b2 (TOPOII\u03b2) and B\u2010cell lymphoma 2 (BCL2) are well known in the development of drug resistance in cancer cells. The aim of this study was to evaluate the relationship between them and the clinicopathological features, their expression differences between tumor tissue and experimental drug\u2010resistant model in tongue carcinoma.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Multiple drug resistance protein 1, LRP, TOPOII\u03b2, and BCL2 expression was examined by immunohistochemistry in specimens from radical surgeries of 65 patients with tongue carcinoma. A cisplatin\u2010resistance cell line, SCC\u201015\/cisplatin, was established from a cisplatin\u2010sensitive cell line, SCC\u201015. A MTT\u2010based method was used to analyze drug potencies. Immunofluorescence was used to detect protein expression in both cell lines. Western blot was used to compare the protein expressions in specimens and SCC\u201015\/cisplatin cells.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> We found higher expression of MRP1, LRP, and BCL2 and lower expression of TOPOII\u03b2 in tongue carcinoma compared with adjacent non\u2010neoplastic tongue tissues (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). In addition, MRP1 and TopoII\u03b2 expression were significantly associated with clinical stage, lymph node metastasis and histologic grade, and LRP was significantly associated with histologic grade in the samples (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Finally, Western blot showed that higher expressions of MRP1, LRP, and BCL2 and lower expression of TopoII\u03b2 were observed in SCC\u201015\/cisplatin cells than in clinical samples.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our results suggest that the high expressions of MRP1, LRP, and BCL2 and low expression of TOPOII\u03b2 in patients with tongue carcinoma indicates that intrinsic drug resistance may exist in tongue carcinoma, and is associated with tumor differentiation and cisplatin resistance in tongue carcinoma.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2011.01066.x","type":"journal-article","created":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T09:23:26Z","timestamp":1311845006000},"page":"141-148","source":"Crossref","is-referenced-by-count":29,"title":["The expression and significance of MRP1, LRP, TOPOII\u03b2, and BCL2 in tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"41","author":[{"given":"Bin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010kuo","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010bin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010zhang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2167"},{"key":"e_1_2_6_3_2","first-page":"559","article-title":"Recent clinical trials using cisplatin, carboplatin and their combination chemotherapy drugs (review)","volume":"11","author":"Boulikas T","year":"2004","journal-title":"Oncol Rep"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568026043387746"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31816e2eb7"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23206"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2737"},{"key":"e_1_2_6_8_2","first-page":"1","article-title":"Cancer multidrug resistance (MDR): a major impediment to effective chemotherapy","volume":"9","author":"Ullah MF","year":"2008","journal-title":"Asian Pac J Cancer Prev"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2003.09.006"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2006.21.2.253"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2010.02.002"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1598"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602958"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.01.060"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.033"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000086984"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/1078155209351329"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2008.00974.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.3816\/CLC.2009.n.078"},{"key":"e_1_2_6_20_2","first-page":"5298","article-title":"Absence of topoisomerase II beta in an amsacrine\u2010resistant human leukemia cell line with mutant topoisomerase II alpha","volume":"58","author":"Herzog CE","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704002104"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2607"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2006.00378.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/joim.1997.242.s740.139","article-title":"Avoidance of apoptosis as a mechanism of drug resistance","volume":"740","author":"Dive C","year":"1997","journal-title":"J Intern Med Suppl"}],"container-title":["Journal of Oral Pathology &amp; 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