{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:34:00Z","timestamp":1781530440954,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T00:00:00Z","timestamp":1330905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p><jats:italic>J Oral Pathol Med<\/jats:italic> (2012) <jats:bold>41<\/jats:bold>: 470\u2013476<\/jats:p><jats:p><jats:bold>Background:\u2002<\/jats:bold> Aberrant expression of homeobox genes (HOX), normally required for the differentiation of a particular tissue, has been reported in several types of cancer, but poorly addressed in oral squamous cell carcinoma (OSCC). The present study investigated the expression of HOXC5 in OSCC and identified molecular biomarker whose expression is associated with the multistep oral carcinogenesis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The expression of HOXC5, proliferation cell nuclear antigen (PCNA), and Bcl\u20102 was examined by RT\u2010PCR and Western blot analysis and confirmed by immunohistochemistry and transferase\u2010mediated dUTP nick end\u2010labeling (TUNEL) assay in a 4\u2010nitroquinoline 1\u2010oxide (4NQO)\u2010induced rat tongue carcinogenesis model.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Homeobox genes C5 was overexpressed in SCC tissues, but not in normal tissues by RT\u2010PCR and Western blot analysis. Along with the progress of multistep carcinogenesis, the levels of HOXC5 expression of mRNA and protein significantly increased during the dysplasia (moderate to severe dysplasia) when compared with normal and hyperplasia. The levels of PCNA and Bcl\u20102 were sequentially increased from hyperplasia to dysplasia and SCC. By immunohistochemistry, HOXC5 expression was significantly increased in dysplasia, whereas PCNA expression was gradually increased during tongue carcinogenesis. TUNEL\u2010positive cells were increased until dysplasia, but reduced in SCC.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> These results indicate that overexpression of HOXC5 is correlated with oral carcinogenesis and strongly contributed to the development of OSCC. HOXC5 may be a useful biomarker and has an emerging therapeutic target of OSCC.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2012.01133.x","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T22:14:09Z","timestamp":1330985649000},"page":"470-476","source":"Crossref","is-referenced-by-count":12,"title":["Homeobox C5 expression is associated with the progression of 4\u2010nitroquinoline 1\u2010oxide\u2010induced rat tongue 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