{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:25:31Z","timestamp":1761863131890},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T00:00:00Z","timestamp":1343001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p><jats:italic>J Oral Pathol Med<\/jats:italic> (2013)<jats:bold>42<\/jats:bold>: 140\u2013147<\/jats:p><jats:p><jats:bold>Background:\u2002<\/jats:bold> The carcinogenesis mechanism of adenoid cystic carcinoma (ACC) of the salivary gland is poorly understood. MicroRNA155 (miRNA155) has been involved in the carcinogenesis of many malignant tumors. The present study aims to examine the role of miRNA155 in tumor growth and invasion of ACC.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> MiRNA155 expression was determined in ACC specimens along with normal salivary glands by quantitative PCR. Using ACC\u20102 cells as a model for ACC, cell proliferation was examined by MTT assay after knocking down miRNA155 expression, and cell cycle analysis was performed. Invasive capacity of ACC\u20102 cells was examined by a Transwell culture assay. The effect of miRNA155 on tumor growth was also examined <jats:italic>in vivo<\/jats:italic> using mouse models. The effect of miRNA155 on epidermal growth factor receptor (EGFR)\/NF\u2010\u03baB was studied by quantitative PCR and electrophoretic mobility shift assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> MiRNA155 was over\u2010expressed in ACC. Proliferation of ACC\u20102 cells was markedly inhibited by knocking down miRNA155, resulting from a blockade of cell cycle in the G1 phase. Inhibition of miRNA155 significantly suppressed the invasive capacity of ACC\u20102 cells. <jats:italic>In vivo<\/jats:italic> growth of ACC\u20102 cell\u2010derived tumors was significantly slower by inhibition of miRNA155. Inhibition of miRNA155 also resulted in decreased expression of EGFR and RelA (NF\u2010\u03baB).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The results suggest that miRNA155 facilitates cell cycle progression and promotes invasion in ACC and that the EGFR\/NF\u2010\u03baB pathway might participate in mediating the effects of miRNA155. This study has provided insights into the carcinogenic mechanisms of ACC and identified new targets for intervention of salivary ACC.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2012.01189.x","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T12:25:10Z","timestamp":1343046310000},"page":"140-147","source":"Crossref","is-referenced-by-count":20,"title":["MicroRNA155 in the growth and invasion of salivary adenoid cystic carcinoma"],"prefix":"10.1111","volume":"42","author":[{"given":"Liu","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjie","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingye","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xihu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.04.020"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.01.001"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00006.2010"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2009.02.013"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1783"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0228"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.08.144"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-0241"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0901-5027(05)80830-6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22758"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101795108"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.07.021"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.11.106"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000231984"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01248-10"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20785"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdj971"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.05.008"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200108000-00011"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-9-101"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210147"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0714.2012.01189.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0714.2012.01189.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T21:21:59Z","timestamp":1697318519000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0714.2012.01189.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,23]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/j.1600-0714.2012.01189.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0714.2012.01189.x","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,23]]}}}