{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:27:38Z","timestamp":1792121258518,"version":"4.4.0"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T00:00:00Z","timestamp":1341187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p><jats:italic>J Oral Pathol Med<\/jats:italic> (2013)<jats:bold>42<\/jats:bold>: 154\u2013161<\/jats:p><jats:p><jats:bold>Background:\u2002<\/jats:bold>  <jats:italic>BCL2L12<\/jats:italic> is a recently identified gene belonging to the <jats:italic>BCL2<\/jats:italic> family, members of which are implicated in head and neck squamous cell carcinoma (HNSCC). We have recently shown that <jats:italic>BCL2L12<\/jats:italic> mRNA expression is an unfavorable prognostic indicator in nasopharyngeal carcinoma (NPC) and that <jats:italic>BCL2L12<\/jats:italic> can be regarded as a novel, useful tissue biomarker for the prediction of NPC patients\u2019 short\u2010term relapse. The aim of this study was to analyze the mRNA expression of the novel apoptosis\u2010related gene <jats:italic>BCL2L12<\/jats:italic> in patients with HNSCC.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Total RNA was isolated from 53 malignant tumors originating in larynx, pharynx, tongue, buccal mucosa, parotid glands, and nasal cavity, as well as from 34 adjacent non\u2010cancerous tissue specimens, resected from patients with HNSCC. A highly sensitive real\u2010time PCR method for <jats:italic>BCL2L12<\/jats:italic> mRNA quantification in head and neck tissues was developed using the SYBR<jats:sup>\u00ae<\/jats:sup> Green chemistry. After preparing cDNA by reverse transcription, relative quantification was performed using the comparative <jats:italic>C<\/jats:italic><jats:sub>T<\/jats:sub> (<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jop1190_mu1.gif\" xlink:title=\"inline image\"\/>) method.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>  <jats:italic>BCL2L12<\/jats:italic> mRNA levels were lower in laryngeal tumors of advanced tumor, node, metastasis (TNM) stage or bigger size and in well\u2010differentiated malignant tongue neoplasms, compared with early\u2010stage laryngeal tumors or poorly differentiated tongue tumors. Interestingly, the <jats:italic>BCL2L12<\/jats:italic> expression showed significant discriminatory value, distinguishing efficiently patients with tongue squamous cell carcinoma (SCC) from non\u2010cancerous population.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> This is the first study examining the <jats:italic>BCL2L12<\/jats:italic> mRNA expression in HNSCC. Our results suggest that <jats:italic>BCL2L12<\/jats:italic> mRNA expression may serve as a potential prognostic biomarker in tongue and\/or larynx SCC, which principally constitute the great majority of HNSCC cases worldwide.<\/jats:p>","DOI":"10.1111\/j.1600-0714.2012.01190.x","type":"journal-article","created":{"date-parts":[[2012,7,2]],"date-time":"2012-07-02T12:30:16Z","timestamp":1341232216000},"page":"154-161","source":"Crossref","is-referenced-by-count":15,"title":["Quantitative expression analysis of the apoptosis\u2010related gene, <i>BCL2L12<\/i>, in head and neck squamous cell carcinoma"],"prefix":"10.1111","volume":"42","author":[{"given":"Panagiota\u2010Aikaterini","family":"Geomela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos 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