{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:15:03Z","timestamp":1778627703096,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T00:00:00Z","timestamp":1415318400000},"content-version":"vor","delay-in-days":6154,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>In order to clarify the initial process of dentin mineralization, the inhibitory effect of 1\u2010hydroxyethylidene\u20101,1\u2010bisphosphonate (HEBP) on dentin mineralization was investigated. Rats (100 g) were subcutaneously injected with HEBP (8 mg P\/kg) for 7 or 14 d, and the incisors were processed for Ca histochemistry and\/or electron microscopy. HEBP\u2010treated incisors demonstrated ladder\u2010like alternate rows of mineralized and non\u2010mineralized dentin at the apical end. GBHA revealed moderate Ca reactions in the non\u2010mineralized circumpulpal dentin matrix where electron microscopy revealed rich distribution of fine mesh\u2010like electron\u2010dense material. Non\u2010mineralized mantle dentin matrix was negative for Ca but contained numerous matrix vesicles (MVs) filled with crystalline and\/or amorphous mineral deposits. Mineralization of circumpulpal dentin occurred independently of MV\u2010rich mantle dentin layer in affected specimens. Our data provide histochemical evidence of <jats:italic>possible<\/jats:italic> Ca\u2010binding property of the circumpulpal dentin matrix and its absence in the mantle dentin where MV\u2010mediated mineralization occurs. In the mantle dentin, HEBP does not interfere with crystal growth in MVs but inhibits its outgrowth after membrane rupture. It is proposed that circumpulpal dentin matrix has a potential to mineralize independently of MV\u2010mediated mineralization of mantle dentin, although MVs determine the initial site and timing of dentin mineralization.<\/jats:p>","DOI":"10.1111\/j.1600-0722.1998.tb02187.x","type":"journal-article","created":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T19:13:38Z","timestamp":1422645218000},"page":"274-281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Demonstration of putative Ca\u2010binding domains in dentin matrix of rat incisors after daily injections of 1\u2010hydroxyethylidene\u20101,1\u2010bisphosphonate (HEBP)"],"prefix":"10.1111","volume":"106","author":[{"given":"Yoshiro","family":"Takano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujiro","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Terashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Ohya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norimasa","family":"Kurosaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,7]]},"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.1998.tb02187.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.1998.tb02187.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.1998.tb02187.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.1998.tb02187.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T03:03:39Z","timestamp":1693969419000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0722.1998.tb02187.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":0,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1111\/j.1600-0722.1998.tb02187.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0722.1998.tb02187.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]},"assertion":[{"value":"2014-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}