{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T05:11:08Z","timestamp":1695877868148},"reference-count":27,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2006,5,2]],"date-time":"2006-05-02T00:00:00Z","timestamp":1146528000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p>Degradation of enamel matrix proteins, and their removal during early maturation, is critical for the growth of large enamel crystals in the subsequent processes of enamel maturation. In this study, we sought to demonstrate, using <jats:italic>in vivo<\/jats:italic> zymography, the exact sites of proteolysis in maturing enamel and its relationship to the overlying ameloblasts. The maturing enamel surfaces of rat and bovine incisors were exposed and painted either with pre\u2010exposed autoradiographic emulsion or with densely fluorescein\u2010conjugated (DQ) gelatin. After a few hours, photographic development of the emulsion revealed alternate black and white banding patterns over the maturing enamel surface. DQ gelatin also revealed similar banding patterns of fluorescent and non\u2010fluorescent regions. White, powdery areas of emulsion and fluorescent bands of DQ gelatin both corresponded to the areas of ruffle\u2010ended ameloblasts, at least up to the mid stages of enamel maturation, implicating a predominant contribution of ruffle\u2010ended ameloblasts in the degradation of enamel matrix proteins. Powdery white bands in autoradiographic emulsion shifted from the areas of ruffle\u2010ended to smooth\u2010ended ameloblasts in late maturation in both bovine and rat incisors and were not influenced by proteinase inhibitors or heat inactivation, implicating non\u2010enzymatic interactions. DQ gelatin, in fact, did not generate any fluorescence in such smooth\u2010ended ameloblast regions.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2006.00295.x","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T15:27:15Z","timestamp":1146756435000},"page":"52-58","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteolysis on maturing enamel surface, as shown by gel\u2010coating methods"],"prefix":"10.1111","volume":"114","author":[{"given":"Yoshiro","family":"Takano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Yamamoto\u2010Shuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Terashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00525-2"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2560965"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345980770020601"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100040101"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2002.21301.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790011301"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3181015"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008208909114129"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90338-3"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/9.5.469"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(70)90109-3"},{"key":"e_1_2_5_13_2","first-page":"61","article-title":"The visualization of the patterns of ameloblast modulation by the glyoxal bis (2\u2010hydroxyanil) staining method","author":"Takano Y","year":"1982","journal-title":"J Dent Res"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00489530"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/38.12.2254645"},{"key":"e_1_2_5_16_2","first-page":"45","article-title":"Ameloblast modulation in the maturation zone of the rat incisor enamel organ. A light and electron microscopic study","volume":"124","author":"Josephsen K","year":"1977","journal-title":"J Anat"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1679\/aohc1950.43.385"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374870010020401"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092370212"},{"key":"e_1_2_5_20_2","first-page":"196","article-title":"Demonstration of Ca2+\u2010ATPase activity in the maturation ameloblast of rat incisor after vascular perfusion","volume":"36","author":"Takano Y","year":"1987","journal-title":"J Electron Microsc (Tokyo)"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/35.4.2950164"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/41.2.7678268"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100022101"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580024101"},{"key":"e_1_2_5_25_2","first-page":"156","article-title":"Enamel maturation","volume":"205","author":"Robinson C","year":"1997","journal-title":"Ciba Found Symp"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(80)90059-2"},{"key":"e_1_2_5_27_2","first-page":"1563","article-title":"Correlation of rat incisor ameloblasts with maturation cycles as displayed on enamel surface with EDTA","volume":"61","author":"Reith EJ","year":"1982","journal-title":"J Dent Res"},{"key":"e_1_2_5_28_2","first-page":"106","article-title":"Histochemical aspects of calcium regulation by the enamel forming cells during matrix formation and maturation","volume":"69","author":"Takano Y.","year":"1994","journal-title":"Kaibogaku Zasshi"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.2006.00295.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.2006.00295.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T06:43:52Z","timestamp":1695797032000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0722.2006.00295.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":27,"journal-issue":{"issue":"s1","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1600-0722.2006.00295.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0722.2006.00295.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]},"assertion":[{"value":"2006-05-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}