{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:32:35Z","timestamp":1780090355971,"version":"3.54.0"},"reference-count":28,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2006,5,2]],"date-time":"2006-05-02T00:00:00Z","timestamp":1146528000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p>We tested the hypothesis that high\u2010calcium medium given prior to or immediately after exposure to fluoride (F) reduces the negative effects of F on secretory amelogenesis. Hamster molar tooth germs were grown in organ culture in media with different calcium levels. Deposition of enamel matrix and matrix mineralization were monitored by incorporation of [<jats:sup>3<\/jats:sup>H]proline and uptake of <jats:sup>45<\/jats:sup>Ca and acid\u2010soluble <jats:sup>32<\/jats:sup>PO<jats:sub>4<\/jats:sub>. Ameloblast structure and the occurrence of a fluorotic enamel matrix were examined by light and electron microscopy. A preculture of explants in high\u2010calcium medium partially prevented the formation of fluorotic (non\u2010mineralizing) enamel matrix, increased matrix secretion but could not prevent F\u2010induced hypermineralization of the pre\u2010exposure enamel. High\u2010calcium medium, applied after F insult, accelerated the recovery of fluorotic matrix, improved ameloblast structure, enhanced amelogenin secretion, and increased enamel thickness. The data indicate that it might be the balance between the amount of mineral deposition and that of matrix secretion which is critical for the mineralization of newly secreted enamel. Exposure to F disturbs this balance by enhancing mineralization of the pre\u2010exposure enamel, probably generating an excess of protons. High calcium may protect against F exposure by enhancing amelogenin secretion into the enamel space, thereby increasing the local buffering capacity at the mineralization front.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2006.00307.x","type":"journal-article","created":{"date-parts":[[2006,5,4]],"date-time":"2006-05-04T15:27:15Z","timestamp":1146756435000},"page":"116-122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of calcium, given before or after a fluoride insult, on hamster secretory amelogenesis <i>in vitro<\/i>"],"prefix":"10.1111","volume":"114","author":[{"given":"Antonius L. J. J.","family":"Bronckers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodorus J. M.","family":"Bervoets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph H. M.","family":"W\u00f6ltgens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donacian M.","family":"Lyaruu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,5,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000260157"},{"key":"e_1_2_5_3_2","first-page":"510","article-title":"Clinical and structural features and possible pathogenic mechanisms of dental fluorosis","volume":"85","author":"Fejerskow O","year":"1977","journal-title":"Scand J Dent Res"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0528.1978.tb01173.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2002.0139"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(84)90171-7"},{"key":"e_1_2_5_7_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1177\/08959374870010020501","article-title":"Studies on the changes in developing enamel caused by ingestion of high levels of fluoride in the rat","volume":"1","author":"Den Besten PK","year":"1987","journal-title":"Adv Dent Res"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02546229"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-7325.1999.tb03277.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/154411130201300206"},{"key":"e_1_2_5_11_2","first-page":"212","article-title":"Micro\u2010PIGE determination of fluorine distribution in developing hamster tooth germs","volume":"3","author":"Lyaruu DM","year":"1989","journal-title":"J Histochem Cytochem"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092370212"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000077766"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(84)90011-6"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(86)90034-8"},{"key":"e_1_2_5_16_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1177\/08959374890030022201","article-title":"X\u2010ray microanalysis of the mineralization patterns in developing enamel in hamster tooth germs exposed to fluoride in vitro during the secretory phase of amelogenesis","volume":"3","author":"Lyaruu DM","year":"1989","journal-title":"Adv Dent Res"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(87)90053-7"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90017-4"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(87)90036-7"},{"key":"e_1_2_5_20_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1177\/08959374870010020801","article-title":"Effect of calcium and phosphate on secretion of enamel matrix and its subsequent mineralization in vitro","volume":"1","author":"W\u00f6ltgens JHM","year":"1987","journal-title":"Adv Dent Res"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60119a033"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(05)80002-2"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/713713501"},{"key":"e_1_2_5_24_2","first-page":"369","article-title":"Distribution in developing rat enamel of simultaneously injected fluoride and calcium","volume":"70","author":"Hammarstr\u00f6m L","year":"1971","journal-title":"Scand J Dent Res"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411950060020701"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.041002"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02555250"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809017038"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199606)245:2<208::AID-AR8>3.0.CO;2-S"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.2006.00307.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.2006.00307.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T06:42:41Z","timestamp":1695796961000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0722.2006.00307.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":28,"journal-issue":{"issue":"s1","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1600-0722.2006.00307.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0722.2006.00307.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]},"assertion":[{"value":"2006-05-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}