{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T05:55:03Z","timestamp":1763704503435},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T00:00:00Z","timestamp":1215561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p>Endothelial monocyte\u2010activating polypeptide II (EMAP\u2010II) is an inflammatory cytokine with chemotactic activity. Because the dental follicle (DF) recruits mononuclear cells (osteoclast precursors) to promote the osteoclastogenesis needed for tooth eruption, it was the aim of this study to determine if EMAP\u2010II contributes to this recruitment. Using a DNA microarray, EMAP\u2010II was found to be highly expressed <jats:italic>in vivo<\/jats:italic> in the DFs of day 1 to day 11 postnatal rats, with its expression elevated on days 1 and 3. Use of a short interfering RNA (siRNA) to knock down EMAP\u2010II expression resulted in a reduction in the expression of colony\u2010stimulating factor\u20101 <jats:bold>(<\/jats:bold>CSF\u20101) and monocyte chemoattractant protein\u20101 <jats:bold>(<\/jats:bold>MCP\u20101) in the DF cells. Addition of EMAP\u2010II protein to the DF cells partially restored the expression of CSF\u20101 and MCP\u20101. In chemotaxis assays using either conditioned medium of the DF cells with anti\u2010(EMAP\u2010II) immunoglobulin G added or conditioned medium of DF cells with EMAP\u2010II knocked down by siRNA, migration indexes of bone marrow mononuclear cells were significantly reduced. These results suggest that EMAP\u2010II is another chemotactic molecule in the dental follicle involved in the recruitment of mononuclear cells, and that EMAP\u2010II may exert its chemotactic function directly by recruiting mononuclear cells and indirectly by enhancing the expression of other chemotactic molecules (CSF\u20101 and MCP\u20101).<\/jats:p>","DOI":"10.1111\/j.1600-0722.2008.00547.x","type":"journal-article","created":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T15:24:45Z","timestamp":1215617085000},"page":"334-340","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression of endothelial monocyte\u2010activating polypeptide II in the rat dental follicle and its potential role in tooth eruption"],"prefix":"10.1111","volume":"116","author":[{"given":"Dawen","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary 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