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Eur J Oral Sci 2010; 118: 168\u2013176. \u00a9 2010 The Authors. Journal compilation \u00a9 2010 Eur J Oral Sci<\/jats:italic> <\/jats:p><jats:p>Invasion is a hallmark of malignancy. The aim of this study was to develop an <jats:italic>in vitro<\/jats:italic> model that can be used for experimental studies of cancer cell invasion. The organotypic oral cancer model was constructed by growing oral squamous cell carcinoma (OSCC) cells on a collagen matrix in which normal human fibroblasts were incorporated. Immunohistochemical staining of the model showed that the expression of invasion\u2010related molecules such as phosphorylated extracellular signal\u2010regulated kinases 1 and 2 (p\u2010ERK1\/2), cyclooxygenase\u20102 (COX\u20102), p75<jats:sup>NTR<\/jats:sup>, and hepatocyte growth factor receptor (Met) was similar to that seen in OSCC. Treatment of the model with cobalt chloride (CoCl<jats:sub>2<\/jats:sub>) to mimic hypoxic conditions increased cancer cell invasion, defined as the appearance of cancer cell islands protruding into the matrix. Models treated with CoCl<jats:sub>2<\/jats:sub> showed increased expression of p75<jats:sup>NTR<\/jats:sup> and laminin\u20105 in the cancer cells, and a more pronounced fragmentation of collagen IV in the basal membrane area, in contrast to models that were left untreated. The results indicate that the present model is well suited for studies on cancer cell invasion in the matrix and that the addition of CoCl<jats:sub>2<\/jats:sub> on day 3 of the experiment is indicated because it markedly increases the invasion and improves the model.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2010.00720.x","type":"journal-article","created":{"date-parts":[[2010,3,11]],"date-time":"2010-03-11T09:57:50Z","timestamp":1268301470000},"page":"168-176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Induction of invasion in an organotypic oral cancer model by CoCl<sub>2<\/sub>, a hypoxia mimetic"],"prefix":"10.1111","volume":"118","author":[{"given":"Ingvild J.","family":"Brusevold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilla","family":"Husvik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olav","family":"Schreurs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl","family":"Schenck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magne","family":"Bryne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tine M.","family":"S\u00f8land","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.4065\/82.7.878"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.11.012"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.07.021"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1858"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.08.015"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2005.05.002"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/35077241"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2067"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2005.00308.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00655.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/stke.4072007cm8"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300463200"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.2.271.271"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/hed.20223"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2005.06.038"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034508329519"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1747.2003.12616.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00656.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03063.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2002.00147.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01295.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1997.5581514.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2004.09.009"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0904-2512.2004.00118.x"},{"key":"e_1_2_6_26_2","unstructured":"WeinbergRAThe biology of cancer. 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