{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:33:16Z","timestamp":1759959196406},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T00:00:00Z","timestamp":1278892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p> <jats:italic>Liu D, Yao S, Wise GE. MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption. Eur J Oral Sci 2010; 118: 333\u2013341. \u00a9 2010 The Authors. Journal compilation \u00a9 2010 Eur J Oral Sci<\/jats:italic> <\/jats:p><jats:p>Myeloid differentiation factor 88 (MyD88) is a key adaptor molecule in the interleukin (IL)\u20101 and IL\u201018 toll\u2010like receptor signaling pathways. Because MyD88 is present in dental follicle (DF) cells <jats:italic>in vitro<\/jats:italic>, the purpose of this study was to determine its chronological expression <jats:italic>in vivo<\/jats:italic>, as well as its possible role in osteoclastogenesis and tooth eruption. An oligo DNA microarray was used to determine expression of the <jats:italic>Myd88<\/jats:italic> gene <jats:italic>in vivo<\/jats:italic> in the DFs from the first mandibular molars of postnatal rats from days 1 to 11. The results showed that <jats:italic>MyD88<\/jats:italic> was expressed maximally on day 3. Using small interfering RNA (siRNA) to knock down MyD88 expression in the DF cells also reduced the expression of the nuclear factor\u2010kappa B\u20101 (<jats:italic>NFKB1<\/jats:italic>) and monocyte chemoattractant protein 1 (<jats:italic>MCP\u20101<\/jats:italic>) genes. Interleukin\u20101\u03b1 up\u2010regulated the expression of <jats:italic>NFKB1<\/jats:italic>, <jats:italic>MCP\u20101<\/jats:italic>, and receptor activator of nuclear factor kappa B ligand (<jats:italic>RANKL<\/jats:italic>), but knockdown of <jats:italic>MyD88<\/jats:italic> nullified this IL\u20101\u03b1 effect. Conditioned medium from DF cells with <jats:italic>MyD88<\/jats:italic> knocked down had reduced chemotactic activity for mononuclear cells and reduced osteoclastogenesis, as opposed to controls. In conclusion, the maximal expression of <jats:italic>MyD88<\/jats:italic> in the DF of postnatal day 3 rats may contribute to the major burst of osteoclastogenesis needed for eruption by up\u2010regulating <jats:italic>MCP\u20101<\/jats:italic> and <jats:italic>RANKL<\/jats:italic> expression.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2010.00751.x","type":"journal-article","created":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T18:52:37Z","timestamp":1279047157000},"page":"333-341","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth 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