{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T22:57:34Z","timestamp":1793228254947,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T00:00:00Z","timestamp":1326412800000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p><jats:italic>Simmer JP, Richardson AS, Smith CE, Hu Y, Hu JC\u2010C. Expression of kallikrein\u2010related peptidase 4 in dental and non\u2010dental tissues.<\/jats:italic>\n          \u2028\n          <jats:italic>Eur J Oral Sci 2011; 119 (Suppl. 1): 226\u2013233. \u00a9 2011 Eur J Oral Sci<\/jats:italic><\/jats:p><jats:p>Kallikrein\u2010related peptidase 4 (KLK4) is critical for proper dental enamel formation. <jats:italic>Klk4<\/jats:italic> null mice, and humans with two defective <jats:italic>KLK4<\/jats:italic> alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liver, and salivary gland. In this study we used the <jats:italic>Klk4<\/jats:italic> knockout\/NLS\u2010<jats:italic>lacZ<\/jats:italic> knockin mouse to assay <jats:italic>Klk4<\/jats:italic> expression using \u03b2\u2010galactosidase histochemistry. Incubations for 5\u2003h were used to detect KLK4 expression with minimal endogenous background, while overnight incubations susceptible to false positives were used to look for trace KLK4 expression. Developing maxillary molars at postnatal days 5, 6, 7, 8, and 14, developing mandibular incisors at postnatal day 14, and selected non\u2010dental tissues from adult wild\u2010type and <jats:italic>Klk4<\/jats:italic><jats:sup><jats:italic>lacZ\/lacZ<\/jats:italic><\/jats:sup> mice were examined by X\u2010gal histochemistry. After 5\u2003h of incubation, X\u2010gal staining was observed specifically in the nuclei of maturation\u2010stage ameloblasts in molars and incisors from <jats:italic>Klk4<\/jats:italic><jats:sup><jats:italic>lacZ\/lacZ<\/jats:italic><\/jats:sup> mice and was detected weakly in the nuclei of salivary gland ducts and in patches of prostate epithelia. We conclude that KLK4 is predominantly a tooth\u2010specific protease with low expression in submandibular salivary gland and prostate, and with no detectable expression in liver, kidney, testis, ovary, oviduct, epididymis, and vas deferens.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2011.00834.x","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T18:56:16Z","timestamp":1326480976000},"page":"226-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Expression of kallikrein\u2010related peptidase 4 in dental and non\u2010dental tissues"],"prefix":"10.1111","volume":"119","author":[{"given":"James P.","family":"Simmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia S.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles 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