{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T11:48:04Z","timestamp":1708516084666},"reference-count":35,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T00:00:00Z","timestamp":1326412800000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p><jats:italic>Hu JC\u2010C, Lertlam R, Richardson AS, Smith CE, McKee MD, Simmer JP. Cell proliferation and apoptosis in enamelin null mice. \u2028Eur J Oral Sci 2011; 119 (Suppl. 1): 329\u2013337. \u00a9 2011 Eur J Oral Sci<\/jats:italic><\/jats:p><jats:p>Enamelin is a secreted glycoprotein that is critical for dental enamel formation. Ameloblasts in enamelin (<jats:italic>Enam<\/jats:italic>) null mice develop atypical features that include the absence of a Tomes\u2019 process, expanded endoplasmic reticulum, apparent loss of polarity, and pooling of extracellular matrix in all directions, including between ameloblasts and the stratum intermedium. We hypothesized that ameloblast pathological changes may be associated with increased cell apoptosis. Our objective was to assess apoptotic activity in maxillary first molars of wild\u2010type, <jats:italic>Enam<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup>, and <jats:italic>Enam<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice at postnatal days 5, 7, 9, 14, and 17. Mouse maxillae were characterized by light microscopy after terminal deoxynucleotidyl transferase (TdT)\u2010mediated biotin\u2013dUTP nick\u2010end labelling (TUNEL) or 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) staining. Following the initial deposition of dentin matrix, ameloblasts became highly dysplastic and no enamel crystal ribbons were deposited. Ameloblast apoptosis was observed in the <jats:italic>Enam<\/jats:italic> null mice starting in the secretory stage and with no apparent alteration in cell proliferation. We conclude that in the absence of enamelin and subsequent shutdown of enamel formation, ameloblasts undergo pathological changes early in the secretory stage that are evident as radically altered cell morphology, detachment from the tooth surface, apoptosis, and formation of ectopic calcifications both outside and inside the dystrophic enamel organ.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2011.00860.x","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T18:56:16Z","timestamp":1326480976000},"page":"329-337","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cell proliferation and apoptosis in enamelin null mice"],"prefix":"10.1111","volume":"119","author":[{"given":"Jan C\u2010C.","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangsiyakorn","family":"Lertlam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia S.","family":"Richardson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc D.","family":"McKee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James P.","family":"Simmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,13]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1999.4130"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404279101"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760110201"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00318054"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809023910"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/154411130301400602"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.31358"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2006.00278.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510365662"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509333180"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.40.12.900"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.9.1069"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.16.1673"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msq098"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-8-246"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2010.1280"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000634"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710565200"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2009.00666.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2010.05.007"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200409077"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.033464"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2009.12.019"},{"key":"e_1_2_7_25_2","first-page":"43","article-title":"On the development of the ameloblasts of the molars of the albino rat, with special reference to the enamel\u2010free areas","volume":"21","author":"Addison WHF","year":"1921","journal-title":"Anat Rec"},{"key":"e_1_2_7_26_2","first-page":"44","article-title":"Ultrastructural and histochemical changes and apoptosis of inner enamel epithelium in rat enamel\u2010free area","volume":"18","author":"Yamamoto H","year":"1998","journal-title":"J Craniofac Genet Dev Biol"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(95)00021-G"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01451574"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.103.Supplement.155"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510375829"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700164"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg234"},{"key":"e_1_2_7_34_2","volume-title":"Gene Reviews","author":"Pfendner EG","year":"2008"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.6.1309"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.10.008"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.2011.00860.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.2011.00860.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T02:15:42Z","timestamp":1694571342000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0722.2011.00860.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":35,"journal-issue":{"issue":"s1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1111\/j.1600-0722.2011.00860.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0722.2011.00860.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]},"assertion":[{"value":"2012-01-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}