{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:03:53Z","timestamp":1787015033580,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T00:00:00Z","timestamp":1326412800000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>\n                    <jats:italic>Lacruz RS, Smith CE, Chen Y\u2010B, Hubbard MJ, Hacia JG, Paine ML. Gene\u2010expression analysis of early\u2010 and late\u2010maturation\u2010stage rat enamel organ \u2028Eur J Oral Sci 2011; 119 (Suppl. 1): 149\u2013157. \u00a9 2011 Eur J Oral Sci<\/jats:italic>\n                  <\/jats:p>\n                  <jats:p>\n                    Enamel maturation is a dynamic process that involves high rates of mineral acquisition, associated fluctuations in extracellular pH, and resorption of extracellular enamel proteins. During maturation, ameloblasts change from having a tall, thin, and highly polarized organization, characteristic of the secretory stage, to having a low columnar and widened morphology in the maturation stage. To identify potential differences in gene expression throughout maturation, we obtained enamel organ epithelial cells derived from the early\u2010 and late\u2010maturation stages of rat incisor and analyzed the global gene\u2010expression profiles at each stage. Sixty\u2010three candidate genes were identified as having potential roles in the maturation process. Quantitative PCR was used to confirm the results of this genome\u2010wide analysis in a subset of genes. Transcripts enriched during late maturation (\n                    <jats:italic>n<\/jats:italic>\n                    \u2003=\u200338) included those associated with lysosomal activity, solute carrier transport, and calcium signaling. Also up\u2010regulated were transcripts involved in cellular responses to oxidative stress, proton transport, cell death, and the immune system. Transcripts down\u2010regulated during the late maturation stage (\n                    <jats:italic>n<\/jats:italic>\n                    \u2003=\u200325) included those with functions related to cell adhesion, cell signaling, and T\u2010cell activation. These results indicate that ameloblasts undergo widespread molecular changes during the maturation stage of amelogenesis and hence provide a basis for future functional investigations into the mechanistic basis of enamel mineralization.\n                  <\/jats:p>","DOI":"10.1111\/j.1600-0722.2011.00881.x","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T13:56:16Z","timestamp":1326462976000},"page":"149-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Gene\u2010expression analysis of early\u2010 and late\u2010maturation\u2010stage rat enamel organ"],"prefix":"10.1111","volume":"119","author":[{"given":"Rodrigo S.","family":"Lacruz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles E.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Bu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Hubbard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph G.","family":"Hacia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Paine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,1,13]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2001.00998.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600806"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(06)74003-0"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(01)00153-6"},{"key":"e_1_2_7_6_2","first-page":"273","article-title":"Stereological analysis of organelle distribution within rat incisor enamel organ at succesive stages of amelogenesis","volume":"125","author":"Smith CE","year":"1984","journal-title":"INSERM"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(90)90084-N"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199812)252:4<519::AID-AR3>3.0.CO;2-I"},{"key":"e_1_2_7_9_2","first-page":"364","article-title":"pH gradients in the developing teeth of young mice from autoradiography of [14C]DMO","volume":"232","author":"Lyman GE","year":"1977","journal-title":"Am J Physiol"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100020701"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411950060020701"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090020101"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-009-9326-7"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.041002"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.5A6821.2005"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092240219"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092250312"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq871"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki475"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-547-3_2"},{"key":"e_1_2_7_22_2","article-title":"Requirements for ion and solute transport, and pH regulation, during enamel maturation","author":"Lacruz RS","journal-title":"J Cell Physiol"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1159\/000324248"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100022501"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2010.955211"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-9-10"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00016.2004"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.62.1.493"},{"key":"e_1_2_7_29_2","article-title":"Time\u2010resolved quantitative analysis of CCK1 receptor\u2010induced intracellular calcium increase","author":"Staljanssens D","year":"2011","journal-title":"Peptides"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.99"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21267"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115188"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1064-5"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33551"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2754"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003692511"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20998"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00382-0"},{"key":"e_1_2_7_39_2","first-page":"3","article-title":"Roles of zinc and metallothionein\u20103 in oxidative stress\u2010induced lysosomal dysfunction, cell death, and autophagy in neurons and astrocytes","volume":"30","author":"Lee S\u2010J","year":"2010","journal-title":"Mol Brain"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2011.03.001"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201000097"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01134.2010"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm2002348"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110040401"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH10-08-0529"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.67"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21280"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.009514"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2001.6556"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2010.07.009"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.07.015"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.06.111"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.12273"},{"key":"e_1_2_7_54_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510369304"},{"key":"e_1_2_7_55_2","first-page":"45","article-title":"Ameloblast modulation in the maturation zone of the rat incisor enamel organ. A light and electron microscopy study","volume":"124","author":"Josephsen K","year":"1977","journal-title":"J Anat"},{"key":"e_1_2_7_56_2","first-page":"381","article-title":"The arrangement of ameloblasts on the surface of maturing enamel of the rat incisor tooth","volume":"133","author":"Reith EJ","year":"1981","journal-title":"J Anat"},{"key":"e_1_2_7_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(81)90107-2"},{"key":"e_1_2_7_58_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00218.2010"},{"key":"e_1_2_7_59_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710565200"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0722.2011.00881.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0722.2011.00881.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T22:14:56Z","timestamp":1694556896000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0722.2011.00881.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":58,"journal-issue":{"issue":"s1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1111\/j.1600-0722.2011.00881.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0722.2011.00881.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]},"assertion":[{"value":"2012-01-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}