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Exocyst subunits are involved in isoproterenol\u2010induced amylase release from rat parotid acinar cells.\u2028Eur J Oral Sci 2012; 120: 123\u2013131. \u00a9 2012 Eur J Oral Sci<\/jats:italic><\/jats:p><jats:p>Exocytosis of secretory granules in parotid acinar cells requires multiple events: tethering, docking, priming, and fusion with a luminal plasma membrane. The exocyst complex, which is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84) that are conserved in yeast and mammalian cells, is thought to participate in the exocytotic pathway. However, to date, no exocyst subunit has been identified in salivary glands. In the present study, we investigated the expression and function of exocyst subunits in rat parotid acinar cells. The expression of mRNA for all eight exocyst subunits was detected in parotid acinar cells by RT\u2010PCR, and Sec6 and Sec8 proteins were localized on the luminal plasma membrane. Sec6 interacted with Sec8 after 5\u2003min of stimulation with isoproterenol. In addition, antibodies to\u2010Sec6 and Sec8 inhibited isoproterenol\u2010induced amylase release from streptolysin O\u2010permeabilized parotid acinar cells. 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