{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:55:54Z","timestamp":1787630154069,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81170928"],"award-info":[{"award-number":["81170928"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81141011"],"award-info":[{"award-number":["81141011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>Periodontal diseases, which are characterized by destruction of the connective tissues responsible for restraining the teeth within the jaw, are the main cause of tooth loss. Periodontal regeneration mediated by human periodontal ligament stem cells (<jats:styled-content style=\"fixed-case\">hPDLSC<\/jats:styled-content>s) may offer an alternative strategy for the treatment of periodontal disease. Dogs are a widely used large\u2010animal model for the study of periodontal\u2010disease progression, tissue regeneration, and dental implants, but little attention has been paid to the identification of the cells involved in this species. This study aimed to characterize stem cells isolated from canine periodontal ligament (<jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s). The <jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s, like <jats:styled-content style=\"fixed-case\">hPDLSC<\/jats:styled-content>s, showed clonogenic capability and expressed the mesenchymal stem cell markers <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>146, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105, but not <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34. After induction of osteogenesis, <jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s showed calcium accumulation in vitro. Moreover, <jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s also showed both adipogenic and chondrogenic potential. Compared with cell\u2010free controls, more cementum\/periodontal ligament\u2010like structures were observed in <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>\u201017\/<jats:styled-content style=\"fixed-case\">SCID<\/jats:styled-content> mice into which <jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s had been transplanted. These results suggest that <jats:styled-content style=\"fixed-case\">cPDLSC<\/jats:styled-content>s are clonogenic, highly proliferative, and have multidifferentiation potential, and that they could be used as a new cellular therapeutic approach to facilitate successful and more predictable regeneration of periodontal tissue using a canine model of periodontal disease.<\/jats:p>","DOI":"10.1111\/j.1600-0722.2012.00975.x","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T13:09:58Z","timestamp":1444741798000},"page":"303-310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Identification of multipotent stem cells from adult dog periodontal ligament"],"prefix":"10.1111","volume":"120","author":[{"given":"Wen\u2010Jun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology Beijing 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