{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T03:23:12Z","timestamp":1782703392590,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,5,11]],"date-time":"2004-05-11T00:00:00Z","timestamp":1084233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>Nitric oxide is a diffusible gaseous mediator generated from <jats:sc>l<\/jats:sc>\u2010arginine by inducible and constitutive nitric oxide synthases. It has been associated with cytotoxic effects. Inflammatory cells and Langerhans cells can express the inducible form of nitric oxide synthase and produce large quantities of nitric oxide. The proximity of these cells to melanocytes could result in melanocyte cell death. We studied melanocyte susceptibility to nitric oxide using the nitric oxide donor compound sodium nitroprusside and nitric oxide released by the Langerhans like cell\u2010line XS\u201052 following stimulation with lipopolysaccharide (LPS). Melanocyte lysis, quantified by chromium release in the presence of sodium nitroprusside was both time and concentration dependent. Co\u2010culture of LPS\u2010stimulated XS cells with melanocytes also resulted in melanocyte cell death. No cell death was observed when melanocytes alone were exposed to LPS. Melanocytes were killed even when the co\u2010cultures were performed across Transwells in which there was no direct contact between XS cells and melanocytes. XS\u2010induced melanocyte death was thus dependent on a diffusible factor consistent with nitric oxide. Cell death was markedly decreased in co\u2010cultures performed in the presence of hemoglobin, a nitric oxide quencher. The possible role that nitric oxide may play in disorders associated with loss of pigmentation is discussed.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2004.00151.x","type":"journal-article","created":{"date-parts":[[2004,5,11]],"date-time":"2004-05-11T08:51:22Z","timestamp":1084265482000},"page":"302-306","source":"Crossref","is-referenced-by-count":5,"title":["Nitric Oxide is Toxic to Melanocytes In Vitro"],"prefix":"10.1111","volume":"17","author":[{"given":"Aurel O.","family":"Iuga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abrar A.","family":"Qureshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan A.","family":"Lerner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,5,11]]},"reference":[{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0592-68"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(05)80006-4"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/327524a0"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(94)90245-3"},{"key":"e_1_2_14_6_2","first-page":"2462","article-title":"Activated murine macrophages induce apoptosis in tumor cells through nitric oxide\u2010dependent or \u2010independent mechanisms","volume":"54","author":"Shijun C","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1246"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.17.8137"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00399088"},{"key":"e_1_2_14_10_2","first-page":"181","article-title":"Relationship between cyclic guanosine 3\u2032,5\u2032\u2010monophosphate formation and relaxation of coronary arterial smooth muscle by glyceryl trinitrate, nitroprusside, nitrite and nitric oxide: effects of methylene blue and methemoglobin","volume":"219","author":"Gruetter C","year":"1981","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00084.x"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00816.x"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199712)183:4<469::AID-PATH931>3.0.CO;2-T"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12330572"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.6.2697"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004030100211"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.1997.tb02748.x"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12343428"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00505.x"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.2165\/00128071-200102030-00006"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1996.tb06963.x"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.45.28052"},{"key":"e_1_2_14_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119206"}],"container-title":["Pigment Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0749.2004.00151.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0749.2004.00151.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T11:12:09Z","timestamp":1697281929000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0749.2004.00151.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,11]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1111\/j.1600-0749.2004.00151.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0749.2004.00151.x","archive":["Portico"],"relation":{},"ISSN":["0893-5785","1600-0749"],"issn-type":[{"value":"0893-5785","type":"print"},{"value":"1600-0749","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5,11]]}}}