{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:38:59Z","timestamp":1791272339801,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,12]],"date-time":"2005-01-12T00:00:00Z","timestamp":1105488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Lipids, particularly sphingolipids, are emerging as novel regulators of cellular activity. A placental total lipid fraction (PTLF), the total lipid prepared from an hydroalcoholic extract of fresh term human placenta, was previously shown to have a pigment\u2010inducing activity in an animal model. The PTLF contains sphingolipids which stimulate DNA synthesis and melanin formation with marked morphological changes in B16F10 melanoma cells. In order to identify the mechanism underlying the increased melanin synthesis, B16F10 cells were treated with PTLF to assess the catalytic activities of tyrosinase (i.e. tyrosine hydroxylase and DOPA oxidase), the key regulatory enzyme of melanin synthesis. Tyrosine hydroxylase (estimated by the release of <jats:sup>3<\/jats:sup>H<jats:sub>2<\/jats:sub>O) as well as DOPA oxidase (measured spectrophotometrically and also in non\u2010denaturing gels), was stimulated significantly by PTLF. Western blot analysis demonstrated an increase in the expression of tyrosinase, tyrosinase related proteins 1 and 2 (TRP1 and TRP2) at the protein level and RT\u2010PCR analysis revealed stimulated transcription of tyrosinase, TRP1 and TRP2 mRNAs in PTLF\u2010treated B16F10 cells. Actinomycin D and cycloheximide, inhibitors of transcription and translation, respectively, inhibited PTLF\u2010induction of tyrosinase activity with a corresponding decrease in melanogenesis. In all cases, the response to PTLF was similar to that induced by <jats:italic>\u03b1<\/jats:italic>\u2010melanocyte stimulating hormone, a well\u2010known stimulator of melanogenesis. Thus, these results provide the basis of action of PTLF stimulated melanogenesis in B16F10 cells showing that this placental extract is a strong inducer of pigmentation at the transcriptional and translational levels.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2004.00193.x","type":"journal-article","created":{"date-parts":[[2005,1,12]],"date-time":"2005-01-12T14:16:14Z","timestamp":1105539374000},"page":"25-33","source":"Crossref","is-referenced-by-count":59,"title":["Human placental lipid induces melanogenesis by increasing the expression of tyrosinase and its related proteins in vitro"],"prefix":"10.1111","volume":"18","author":[{"given":"Shampa","family":"Mallick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinmoy","family":"Sarkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidisha","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjan","family":"Bhadra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,12]]},"reference":[{"key":"e_1_2_23_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1789"},{"key":"e_1_2_23_3_1","unstructured":"Bhadra R. Pal P. Roy R. andDutta A.K.(1994).Process for the preparation of an extract from human placenta containing glycosphingolipids and endothelin\u2010like consitituent peptides useful for the treatment of vitiligo. Indian patent appl. no. 1228\/Del\/94 (1994); US patent no. 5690966 25 Nov. 1997; European Union patent appl. no. EP 0839535 6 May 1998 Japanese patent pending"},{"key":"e_1_2_23_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(90)90097-R"},{"key":"e_1_2_23_5_1","first-page":"1451","volume-title":"Dermatology in General Medicine","author":"Gange R.W.","year":"1971"},{"key":"e_1_2_23_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.14.1752358"},{"key":"e_1_2_23_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-0749.2003.00097.x"},{"key":"e_1_2_23_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01534876"},{"key":"e_1_2_23_9_1","first-page":"47","article-title":"Activation of cAMP pathway by \u03b1\u2010Melanotropin mediates the response of human melanocytes to UV\u2010B radiation","volume":"58","author":"Im S.","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_23_10_1","first-page":"33","volume-title":"Pigmentation and Pigmentary Disorders","author":"Ito S.","year":"1993"},{"key":"e_1_2_23_11_1","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/S0021-9258(17)35295-X","article-title":"Tyrosinases from two different loci are expressed by normal and by transformed melanocytes","volume":"266","author":"Jim\u00e9nez M.","year":"1991","journal-title":"J Biol Chem."},{"key":"e_1_2_23_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1993.tb00621.x"},{"key":"e_1_2_23_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(02)00024-4"},{"key":"e_1_2_23_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00366"},{"key":"e_1_2_23_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06925.x"},{"key":"e_1_2_23_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6810464"},{"key":"e_1_2_23_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_23_18_1","doi-asserted-by":"crossref","first-page":"16626","DOI":"10.1016\/S0021-9258(18)66613-X","article-title":"Tyrosinase isozyme heterogeneity in differentiating B16\/C3 melanoma","volume":"261","author":"Laskin J.D.","year":"1986","journal-title":"J Biol Chem."},{"key":"e_1_2_23_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1552"},{"key":"e_1_2_23_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81661-X"},{"key":"e_1_2_23_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_23_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02704913"},{"key":"e_1_2_23_23_1","first-page":"1552","article-title":"Mitogenic and melanogenic activity of human placental protein\/peptides on melanoma cell","volume":"78","author":"Mandal S.K.","year":"2000","journal-title":"Curr Sci."},{"key":"e_1_2_23_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.7.3967"},{"key":"e_1_2_23_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-0749.2003.00113.x"},{"key":"e_1_2_23_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00204.x"},{"key":"e_1_2_23_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1307"},{"key":"e_1_2_23_28_1","first-page":"213","volume-title":"De\u2010lipidation of Plasma Lipoproteins. Methods in Enzymology","author":"Osborne J.C.","year":"1986"},{"key":"e_1_2_23_29_1","volume-title":"Hydroalcoholic Extract of Human Placenta. Its Composition and Melanogenic Relevance","author":"Pal P.","year":"1994"},{"key":"e_1_2_23_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1995.tb04383.x"},{"key":"e_1_2_23_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.2002.01524.x"},{"key":"e_1_2_23_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(91)90003-Y"},{"key":"e_1_2_23_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12482134"},{"key":"e_1_2_23_34_1","first-page":"571","article-title":"Studies on the Cloudman melanoma cell line as a model for the action of MSH","volume":"58","author":"Pawelek J.M.","year":"1985","journal-title":"Yale J Biol Med."},{"key":"e_1_2_23_35_1","doi-asserted-by":"crossref","first-page":"5308","DOI":"10.1016\/S0021-9258(18)99429-9","article-title":"3,4 Dihydroxy\u20101\u2010phenylamine as the tyrosinase cofactor","volume":"242","author":"Pomeranaz S.H.","year":"1965","journal-title":"J Biol Chem."},{"key":"e_1_2_23_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-565970-3.50007-8"},{"key":"e_1_2_23_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90327-1"},{"key":"e_1_2_23_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R200007200"},{"key":"e_1_2_23_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1999.4090"},{"key":"e_1_2_23_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.1.155"},{"key":"e_1_2_23_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00589-7"}],"container-title":["Pigment Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0749.2004.00193.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0749.2004.00193.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T21:14:18Z","timestamp":1696972458000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0749.2004.00193.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,12]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1111\/j.1600-0749.2004.00193.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0749.2004.00193.x","archive":["Portico"],"relation":{},"ISSN":["0893-5785","1600-0749"],"issn-type":[{"value":"0893-5785","type":"print"},{"value":"1600-0749","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1,12]]}}}