{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:48:55Z","timestamp":1778770135215,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,22]],"date-time":"2005-02-22T00:00:00Z","timestamp":1109030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Tyrosinase, the rate\u2010limiting enzyme of melanin synthesis, is a di\u2010copper metalloprotein that catalyzes the conversion of <jats:sc>l<\/jats:sc>\u2010tyrosine to <jats:sc>l<\/jats:sc>\u2010DOPAquinone. Phenylthiourea (PTU) is a well\u2010known inhibitor of tyrosinase and melanin synthesis and is known to interact with sweet potato catechol oxidase, an enzyme possessing copper binding domain homology to tyrosinase. While PTU is frequently used to induce hypopigmentation in biological systems, little is known about its effects on tyrosinase and other melanogenic proteins. We have found that PTU induces degradation of tyrosinase but not of other melanogenic proteins including the tyrosinase\u2010related metalloproteins tyrosinase\u2010related protein (Tyrp)1 and Tyrp2. Using pulse\u2010chase analysis coupled with glycosidase digestion, we observed that tyrosinase degradation occurs following complete maturation of the protein and that degradation was reversed by cysteine protease inhibitor E64 but not proteasome inhibitor N\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010leucinyl\u2010<jats:sc>l<\/jats:sc>\u2010leucinyl\u2010<jats:sc>l<\/jats:sc>\u2010norleucinal. We conclude that PTU specifically induces tyrosinse degradation following Golgi maturation. Our data suggest that in addition to well\u2010known ER\u2010directed quality control, tyrosinase is also subject to post\u2010Golgi quality control.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2005.00213.x","type":"journal-article","created":{"date-parts":[[2005,3,10]],"date-time":"2005-03-10T00:05:27Z","timestamp":1110413127000},"page":"122-129","source":"Crossref","is-referenced-by-count":66,"title":["Degradation of tyrosinase induced by phenylthiourea occurs following Golgi maturation"],"prefix":"10.1111","volume":"18","author":[{"given":"Andrea M.","family":"Hall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seth J.","family":"Orlow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313701200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.31102.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.26.15798"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1242\/dev.105.2.379","article-title":"Cloned mouse melanocyte lines carrying the germline mutations albino and brown: complementation in culture","volume":"126","author":"Bennett D.C.","year":"1989","journal-title":"Development"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.12281"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.4.2139"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-02-0022"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309673200"},{"key":"e_1_2_6_10_1","first-page":"2211","volume-title":"Disorders of Copper Transport","author":"Danks D.M.","year":"1995"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-40-3-123"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.3.409"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)80010-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0021-9258(17)41184-7","article-title":"Studies on the mechanism of action of thiourea and related compounds. 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