{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:18:54Z","timestamp":1792174734018,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T00:00:00Z","timestamp":1108598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>ATP\u2010binding cassette (ABC) transporters play a pivotal role in physiology and pathology. We identified and cloned two novel mRNA isoforms (<jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic> and <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b2<\/jats:italic>) of the ABC transporter <jats:italic>ABCB<\/jats:italic>\u200a5 in human melanoma cells. The deduced <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic> protein appears to be an altered splice variant containing only a putative ABC, whereas the <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b2<\/jats:italic> isoform shares approximately 70% similarity with <jats:italic>ABCB<\/jats:italic>1 (<jats:italic>MDR<\/jats:italic>1) and has a deduced topological arrangement similar to that of the whole carboxyl terminal half of the <jats:italic>ABCB<\/jats:italic>1 gene product, P\u2010glycoprotein, including an intact ABC. Northern blot, real\u2010time PCR, and conventional RT\u2010PCR were used to verify the expression profiles of <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic>. We found that the melanomas included among the NCI\u201060 panel of cell lines preferentially expressed both <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic> and <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b2<\/jats:italic>. However, <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic> expression was undetectable in two amelanotic melanomas (M14 and LOX\u2010IMVI). The expression profile of <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic> in all of the other melanomas of the panel was confirmed both by RT\u2010PCR and by sequencing. Neither <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic> nor <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b2<\/jats:italic> expression was found in normal tissues such as liver, spleen, thymus, kidney, lung, colon, small intestines or placenta. <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic> mRNAs were also expressed in normal melanocytes and in retinal pigment epithelial cells, suggesting that <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic> expression is pigment cell\u2010specific and might be involved in melanogenesis. Our findings indicate that expression of <jats:italic>ABCB<\/jats:italic>\u200a5<jats:italic>\u03b1<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic> might possibly provide two novel molecular markers for differential diagnosis of melanomas and constitute potential molecular targets for therapy of melanomas.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2005.00214.x","type":"journal-article","created":{"date-parts":[[2005,3,10]],"date-time":"2005-03-10T00:05:27Z","timestamp":1110413127000},"page":"102-112","source":"Crossref","is-referenced-by-count":74,"title":["Principal expression of two mRNA isoforms (<i>ABCB\u200a 5\u03b1<\/i> and <i>ABCB\u200a 5\u03b2<\/i> ) of the ATP\u2010binding cassette transporter gene <i>ABCB<\/i>\u200a5 in melanoma cells and melanocytes"],"prefix":"10.1111","volume":"18","author":[{"given":"Kevin G.","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gergely","family":"Szak\u00e1cs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Philippe","family":"Annereau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Rouzaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing\u2010Jie","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio C.","family":"Valencia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrasekharam N.","family":"Nagineni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Hooks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent J.","family":"Hearing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Gottesman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,3,9]]},"reference":[{"key":"e_1_2_16_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0397-236"},{"key":"e_1_2_16_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/11914"},{"key":"e_1_2_16_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.102301.093055"},{"key":"e_1_2_16_5_1","first-page":"2.9.1","volume-title":"Current Protocols in Molecular Biology","author":"Brown T.","year":"1993"},{"key":"e_1_2_16_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90141-K"},{"key":"e_1_2_16_7_1","first-page":"4980","article-title":"Prevalence of multidrug resistance related to activation of the mdr1 gene in human sarcoma mutants derived by single\u2010step doxorubicin selection","volume":"54","author":"Chen G.","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_16_8_1","first-page":"2029","article-title":"Identification of a sister gene to P\u2010glycoprotein","volume":"55","author":"Childs S.","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_16_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/38.9.1974900"},{"key":"e_1_2_16_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012823120935"},{"key":"e_1_2_16_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)31588-1"},{"key":"e_1_2_16_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/mp.55.5.294"},{"key":"e_1_2_16_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.1.265"},{"key":"e_1_2_16_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308700200"},{"key":"e_1_2_16_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.10046"},{"key":"e_1_2_16_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123841"},{"key":"e_1_2_16_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc706"},{"key":"e_1_2_16_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200043489199"},{"key":"e_1_2_16_19_1","first-page":"4091","article-title":"Multidrug (pleiotropic) resistance in doxorubicin\u2010selected variants of the human sarcoma cell line MES\u2010SA","volume":"45","author":"Harker W.G.","year":"1985","journal-title":"Cancer Res"},{"key":"e_1_2_16_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3884"},{"key":"e_1_2_16_21_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.2.230"},{"key":"e_1_2_16_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.23.17536"},{"key":"e_1_2_16_23_1","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/cdli.1.5.569-577.1994","article-title":"Synergistic effects of gamma interferon on inflammatory mediators that induce interleukin\u20106 gene expression and secretion by human retinal pigment epithelial cells","volume":"1","author":"Nagineni C.N.","year":"1994","journal-title":"Clin. 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