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Placental total lipid fraction (PTLF), prepared from a hydroalcoholic extract of fresh term human placenta contains sphingolipids and was recently shown to stimulate melanogenesis via up\u2010regulation of the key enzyme tyrosinase in B16F10 mouse melanoma cells. How such lipids mediate their effects on pigmentation and <jats:italic>tyrosinase<\/jats:italic> expression is a particularly important aspect of melanogenesis. To study the signaling that leads to <jats:italic>tyrosinase<\/jats:italic> expression, we have investigated the roles of the MAPK and Akt\/PKB pathways in B16F10 melanoma cells in melanogenesis in response to PTLF. Treatment of cells with PTLF led to the time dependent phosphorylation of p38 MAPK. SB203580, a p38 MAPK inhibitor, completely blocked the PTLF\u2010induced melanogenesis by inhibiting promoter activity and subsequent expression of tyrosinase. Phosphatidylinositol 3\u2010kinase (PI3K) inhibitor, LY294002 a blocker of the Akt signaling pathway, or an inhibitor of MEK (MAPK\/ERK Kinase), PD98059 when included along with PTLF was found to potentiate PTLF\u2010induced phosphorylation of p38 MAPK together with tyrosinase expression and melanogenesis. The results suggest that the activation of p38 MAPK plays a crucial role in PTLF\u2010induced B16F10 melanogenesis by up\u2010regulating <jats:italic>tyrosinase<\/jats:italic> expression.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2005.00219.x","type":"journal-article","created":{"date-parts":[[2005,3,10]],"date-time":"2005-03-10T00:05:27Z","timestamp":1110413127000},"page":"113-121","source":"Crossref","is-referenced-by-count":71,"title":["Human placental lipid induces melanogenesis through p38 MAPK in B16F10 mouse melanoma"],"prefix":"10.1111","volume":"18","author":[{"given":"Suman Kumar","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinmoy","family":"Sarkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shampa","family":"Mallick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidisha","family":"Saha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabindranath","family":"Bera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjan","family":"Bhadra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,3,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.3.827"},{"key":"e_1_2_11_3_1","unstructured":"Bhadra R. 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