{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:22:15Z","timestamp":1791145335933,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,7,30]],"date-time":"2007-07-30T00:00:00Z","timestamp":1185753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Common generalized vitiligo is an acquired depigmenting disorder characterized by a chronic and progressive loss of melanocytes from the epidermis and hair follicles. We previously proposed a new theory that vitiligo involves the chronic detachment and transepidermal loss of melanocytes caused by autoimmune, neural and impaired redox mechanisms associated with mechanical trauma. In this study, we reconstructed epidermis on dead de\u2010epidermized dermis with normal and\/or non\u2010segmental non\u2010lesional vitiligo (NSV) cells and tested catecholamines or sera or hydrogen peroxide. Under unstressed conditions, the number of melanocytes located in the basal layer was significantly lower in reconstructs made with melanocytes from non\u2010lesional NSV skin and normal keratinocytes compared with controls made with autologous normal melanocytes. The number of non\u2010lesional NSV melanocytes was even lower in reconstructs made with keratinocytes from non\u2010lesional NSV skin. Epinephrine and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> could trigger the transepidermal loss of normal and vitiligo melanocytes. Some sera induced melanocyte detachment but without any clear correlation with disease activity in the donors. In conclusion, our results are the first step to obtaining a reproducible melanocytorrhagic model in vitro with some of the stressors investigated. They support the hypothesis that NSV melanocytes have an intrinsic defect, which limits their adhesion in a reconstructed epidermis, with an enhancer effect of the vitiligo keratinocyte milieu.<\/jats:p>","DOI":"10.1111\/j.1600-0749.2007.00396.x","type":"journal-article","created":{"date-parts":[[2007,7,30]],"date-time":"2007-07-30T11:46:27Z","timestamp":1185795987000},"page":"385-393","source":"Crossref","is-referenced-by-count":67,"title":["The melanocytorrhagic hypothesis of vitiligo tested on pigmented, stressed, reconstructed epidermis"],"prefix":"10.1111","volume":"20","author":[{"given":"M.","family":"Cario\u2010Andr\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Gauthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ta\u00efeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,30]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"931","article-title":"The reconstructed epidermis with melanocytes: a new tool to study pigmentation and photoprotection","volume":"45","author":"Cario\u2010Andr\u00e9 M.","year":"1999","journal-title":"Cell. 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