{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T04:50:57Z","timestamp":1770699057657,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T00:00:00Z","timestamp":1151625600000},"content-version":"vor","delay-in-days":2921,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:p>Adhesion of peripheral blood neutrophils from 5 patients with localized juvenile periodontitis (LJP) and age\u2010 and gender\u2010matched healthy controls was measured using a semi\u2010automated 96\u2010well microtiter plate assay method. Both unstimulated and formyl\u2010methionyl\u2010leucyl\u2010phenylalanine (FMLP, 10\u20131000 nM)\u2010stimulated neutrophils from LJP patients showed in general higher adhesion than did their controls. After 15\u201360 min incubation with 100 and 1000 nM FMLP the numbers of adherent cells were significantly (p&lt;0.05), 2.1\u20132.6\u2010fold higher in LJP patients than in controls. Neutrophils from these LJP patients showed also enhanced respiratory burst activity in response to unopsonized zymosan stimulation. To test whether a decrease in intracellular diacylglycerol (DAG) kinase activity could account for the increased neutrophil adhesion of LJP patients normal neutrophils were treated with R59949 (10 \u03bcM), a DAG\u2010kinase inhibitor. Both unstimulated and FMLP\u2010stimulated normal neutrophiis showed significantly (p&lt;0.05) enhanced adhesion after R59949\u2010treatment. Taken together, our data indicate that neutrophils from the 5 LJP patients investigated here exhibit 2 parallel hyperactivities, namely increased adhesion and enhanced production of reactive oxygen species. Furthermore, our present and previous (Hurttia <jats:italic>et al, J Periodont Res<\/jats:italic> 1997; 32: 401\u2010407) results suggest that the observed neutrophil functional abnormalities in some LJP patients may be associated with decreased cellular DAG\u2010kinase activity. It is proposed that the hyperadherent and \u2010active neutrophils may promote the development of LJP by causing tissue damage in the periodontium.<\/jats:p>","DOI":"10.1111\/j.1600-0765.1998.tb02202.x","type":"journal-article","created":{"date-parts":[[2006,7,2]],"date-time":"2006-07-02T07:23:12Z","timestamp":1151824992000},"page":"292-297","source":"Crossref","is-referenced-by-count":12,"title":["Increased adhesion of peripheral blood neutrophils from patients with localized juvenile 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