{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:31:02Z","timestamp":1773149462947,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,12,6]],"date-time":"2004-12-06T00:00:00Z","timestamp":1102291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Keratinocyte growth factor\u20101 (KGF\u20101) is up\u2010regulated in chronic inflammation and specifically stimulates epithelial cell proliferation by signaling through the epithelial\u2010specific keratinocyte growth factor receptor (KGFR). We examined KGF\u20101 and KGFR protein and gene expression in healthy and diseased periodontal tissues.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Tissues were collected from patients with periodontal health or disease, immediately frozen and stained for KGF\u20101 and KGFR protein expression. Laser capture microdissection of epithelial and connective tissue cells with reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) examined KGF\u20101 and KGFR gene expression profiles and enzymatic digestion with heparitinase, chondroitinase ABC or pre\u2010treatment with suramin examined epithelial surface molecule interactions with KGF\u20101.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> In tissues collected from healthy patients, KGF\u20101 protein localized to areas of junctional and basal oral epithelial cells and was significantly increased in periodontal pocket epithelium (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.01) and in the oral epithelium (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.05) of disease\u2010associated tissues. KGFR localized to the junctional and the parabasal cells of oral epithelium, with the relative staining intensity being increased in disease\u2010associated pocket epithelium (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.05). Laser capture microdissection with RT\u2013PCR confirmed KGF\u20101 and KGFR were specifically expressed by connective tissue and epithelium, respectively. KGF\u20101 localization to epithelial cells was largely eliminated by suramin pre\u2010treatment, indicating interaction with the KGFR.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> KGF\u20101 and KGFR proteins are expressed in healthy periodontal tissues but significantly increased in diseased periodontal tissues. We hypothesize up\u2010regulation of KGF\u20101 and KGFR protein associated with disease regulates epithelial cell behavior associated with onset and progression of periodontal pocket formation.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2004.00780.x","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T16:22:37Z","timestamp":1109175757000},"page":"118-128","source":"Crossref","is-referenced-by-count":23,"title":["Keratinocyte growth factor\u20101 expression in healthy and diseased human periodontal tissues"],"prefix":"10.1111","volume":"40","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James D.","family":"Firth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward E.","family":"Putnins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"key":"e_1_2_15_2_2","first-page":"170","volume-title":"Oral biology at the turn of the century.","author":"Birkedal\u2010Hansen.","year":"1998"},{"key":"e_1_2_15_3_2","first-page":"235","article-title":"Pathogenesis of inflammatory periodontal disease. 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