{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T10:20:47Z","timestamp":1783160447341,"version":"3.54.6"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T00:00:00Z","timestamp":1109116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> Enamel matrix\u2010derived proteins (EMD) are thought to trigger the formation of acellular extrinsic fibre cementum (AEFC), while other reports indicate that EMD may have osteogenic potential. The aim of the present study was to characterize the tissues developing on the root surface following application of EMD.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Twelve human periodontitis\u2010affected teeth, scheduled for extraction, were treated with EMD. Two to 6\u2003weeks later, the teeth were extracted, demineralized and processed for embedding in acrylic and epoxy resins. New tissue formation was analysed by light and transmission electron microscopy.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> New tissue formation on the root was observed in the notch and on both scaled and unscaled root surfaces distant of the notch area in six defects. The newly formed tissues on the root were thick, collagenous, devoid of extrinsic fibres, and had an irregular surface contour. The presence of electron\u2010dense, organic material in the collagenous matrix indicated at least partial mineralization. Embedded cells were numerous and the cells on the matrix surface were very large in size. Abundant rough endoplasmic reticulum and a prominent Golgi complex were evident. The presence of a split between the treated root surfaces and the newly formed tissue was a common observation, as was the presence of bacteria and host cells in the interfacial gap.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Following treatment with EMD, a bone\u2010like tissue resembling cellular intrinsic fibre cementum may develop on the root surfaces, instead of AEFC. Furthermore, EMD may both induce <jats:italic>de\u2003novo<\/jats:italic> formation of a mineralized connective tissue on scaled root surfaces and stimulate matrix deposition on old native cementum. Interfacial bonding appeared to be weak after 6\u2003weeks of healing.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00785.x","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T16:22:37Z","timestamp":1109175757000},"page":"158-167","source":"Crossref","is-referenced-by-count":63,"title":["Effects of enamel matrix proteins on tissue formation along the roots of human teeth"],"prefix":"10.1111","volume":"40","author":[{"given":"Dieter D.","family":"Bosshardt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Sculean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Windisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjarni 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