{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:01:29Z","timestamp":1761764489870},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,20]],"date-time":"2005-06-20T00:00:00Z","timestamp":1119225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Although herpes viruses have been implicated in the pathogenesis of chronic and aggressive periodontitis, few data in the literature refer to quantification of these viruses in periodontal sites, especially in relation to serological findings.<\/jats:p><jats:p><jats:bold>Objective:\u2002<\/jats:bold> The aim of the present study was to compare Epstein\u2013Barr virus (EBV) DNA load in subgingival specimens from chronic periodontitis patients and in periodontally healthy subjects, in relation to serologic testing of IgM and IgG antibodies to EBV.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> A total of 22 chronic periodontitis patients and 13 controls participated in the present study. Seventy\u2010nine subgingival specimens (one pooled, one from a deep and one from a shallow site), sampled with paper points, were analysed with real\u2010time polymerase chain reaction for EBV. Subjects were also examined for anti\u2010EBV IgG and IgM levels in serum, using an enzyme\u2010linked immunosorbent assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> One subject was seronegative for EBV. Three subjects (one patient and two controls) displayed anti\u2010EBV IgM. Their data were excluded from further analysis. All three displayed EBV in their subgingival samples. Nine out of the remaining 20 chronic periodontitis patients and 10 out of 11 controls did not display EBV subgingivally. A statistically significant difference in viral load was observed between pooled and shallow\u2010pocket samples from periodontitis patients but not between samples from deep and shallow pockets (Kruskall\u2013Wallis <jats:sc>anova<\/jats:sc>, Dunn's multiple comparisons test).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Data from the present study do not strongly support the pathogenetic significance of EBV in chronic periodontitis lesions. The data do, however, suggest that parallel serological assessments provide a useful insight into the association of viruses with periodontal disease.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00796.x","type":"journal-article","created":{"date-parts":[[2005,6,20]],"date-time":"2005-06-20T12:58:41Z","timestamp":1119272321000},"page":"294-298","source":"Crossref","is-referenced-by-count":27,"title":["Real\u2010time polymerase chain reaction quantification of Epstein\u2013Barr virus in chronic periodontitis patients"],"prefix":"10.1111","volume":"40","author":[{"given":"Antonis","family":"Konstantinidis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitra","family":"Sakellari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Papa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonis","family":"Antoniadis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2000.035001003.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302x.2000.150501.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.5.478"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00183.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00199.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2000.035001017.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028009879.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00707.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.3986-3992.2002"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(03)00121-5"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302x.2000.150103.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2005.00796.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2005.00796.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T06:44:21Z","timestamp":1697006661000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2005.00796.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6,20]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,8]]}},"alternative-id":["10.1111\/j.1600-0765.2005.00796.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2005.00796.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6,20]]}}}