{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:12:08Z","timestamp":1760865128875,"version":"3.32.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,7]],"date-time":"2005-03-07T00:00:00Z","timestamp":1110153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold>The immunosuppressive drug cyclosporin A has been shown to induce cementum deposition<jats:italic>in\u2003vivo<\/jats:italic>in experimental animals. Using cementoblastoma\u2010derived cells, we have studied whether this drug will be useful to study cementum mineralization and differentiation<jats:italic>in\u2003vitro.<\/jats:italic><\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>Human cementoblastoma cells and gingival fibroblasts (controls) were cultured and treated with 0.5, 1.0 and 5.0\u2003\u00b5g\/ml of cyclosporin A. Cell proliferation was evaluated by MTT (tetrazolium) assay and cell number, and cell viability was assessed by trypan blue dye exclusion. Induction of mineralization was evaluated by alizarin red S staining to detect mineralized nodules and by reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) to assess the expression of bone differentiation markers alkaline phosphatase, osteocalcin, bone sialoprotein and core\u2010binding factor a1 (Cbfa1).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>Cyclosporin A at 5.0\u2003\u00b5g\/ml concentration reduced significantly the increase in the number of cementoblastoma cells. A dose\u2010dependent increase in the number of mineralized nodules occurred in cultures of cementoblastoma\u2010derived cells treated with cyclosporin A, and RT\u2013PCR analyses showed significantly higher levels of expression of alkaline phosphatase, bone sialoprotein, type I collagen, matrix metalloproteinase\u20101, osteocalcin, osteopontin, and Cbfa1. Human gingival fibroblast proliferation and cell number were not affected. Mineralized nodules were not detected in gingival fibroblasts and bone specific proteins were not expressed.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>Presence of cyclosporin A during 14\u2010day culture period appears to suppress the proliferation of cementoblastoma cells and induce the formation mineralized\u2010like tissue by these cells.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00797.x","type":"journal-article","created":{"date-parts":[[2005,4,22]],"date-time":"2005-04-22T16:00:49Z","timestamp":1114185649000},"page":"218-224","source":"Crossref","is-referenced-by-count":9,"title":["Cyclosporin A promotes mineralization by human cementoblastoma\u2010derived cells in culture"],"prefix":"10.1111","volume":"40","author":[{"given":"Higinio","family":"Arzate","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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