{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:38:09Z","timestamp":1790843889432,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,7,4]],"date-time":"2005-07-04T00:00:00Z","timestamp":1120435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Reactive oxygen species (ROS) are implicated in the destruction of the periodontium during inflammatory periodontal diseases. The imbalance in oxidant\/antioxidant activity may be a key factor in the damaging effects of ROS. This study aimed to determine the lipid peroxidation levels in gingival crevicular fluid and saliva, and glutathione (GSH) and glutathione peroxidase (GPx) in saliva in patients with chronic periodontitis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Gingival crevicular fluid and saliva were collected from 13 patients and 9 healthy control subjects during the preliminary study, and from 21 patients during the subsequent study. Lipid peroxidation level, GSH level and GPx activity were determined by spectrophotometric assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The preliminary study found that when comparing patients to healthy controls, the gingival crevicular fluid samples produced the following results, respectively: higher lipid peroxidation concentration (\u00b5<jats:sc>m<\/jats:sc>) (by sites: 167.55 vs. 53.71, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.0001; by subjects: 151.99 vs. 50.66, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.005) and total amount (pmol) (by sites: 93.02 vs. 8.47, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.0001, by subjects: 80.44 vs. 7.84, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.0005). In saliva samples, lower GSH concentration (\u00b5<jats:sc>m<\/jats:sc>) (373.04 vs. 606.67, <jats:italic>p\u2003&lt;<\/jats:italic>\u20030.05), higher lipid peroxidation concentration (\u00b5<jats:sc>m<\/jats:sc>) (0.66 vs. 0.13, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.0005), and no difference in GPx activity were found in patients than in those of healthy controls. The subsequent study showed statistically significant (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.05) improvement of clinical periodontal parameters (plaque index, gingival index, probing attachment level, probing pocket depth and gingival crevicular fluid volume), decreases in gingival crevicular fluid lipid peroxidation levels (concentration and total amount) at the sites after the completion of phase 1 periodontal treatment. Similarly, the periodontal treatment resulted in a significant decrease of lipid peroxidation concentrations (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.05), increase in GSH concentration (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.001), and no change in GPx activity in saliva samples.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The increased levels of lipid peroxidation may play a role in the inflammation and destruction of the periodontium in periodontitis.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00818.x","type":"journal-article","created":{"date-parts":[[2005,8,9]],"date-time":"2005-08-09T16:40:03Z","timestamp":1123605603000},"page":"378-384","source":"Crossref","is-referenced-by-count":217,"title":["Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis"],"prefix":"10.1111","volume":"40","author":[{"given":"C. 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