{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:57:32Z","timestamp":1783537052326,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,9,13]],"date-time":"2005-09-13T00:00:00Z","timestamp":1126569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>Objective:\u2002<\/jats:bold> This study aimed to investigate the expression patterns of the newly discovered human \u03b2\u2010defensin\u20103 (hBD\u20103) in human gingiva.<\/jats:p><jats:p><jats:bold>Background:\u2002<\/jats:bold> Human \u03b2\u2010defensins (hBDs) are a group of small, broad\u2010spectrum, cationic antimicrobial peptides. Our recent study showed that the expression levels of hBD\u20101 and 2 peptides were associated with periodontal conditions.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> A total of 49 gingival biopsies were collected, including 33 samples from 21 patients with chronic periodontitis and 16 samples from 16 periodontally healthy subjects. The expression of hBD\u20103 was detected by immunohistochemistry and <jats:italic>in\u2003situ<\/jats:italic> hybridization. Double staining was undertaken to identify hBD\u20103 peptide\u2010positive cells, using CD\u20101a and cytokeratin 20 as markers for Langerhans cells and Merkel cells, respectively.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> hBD\u20103 peptide was detected in 88% of the samples, which was confined to the gingival epithelia. In healthy control subjects, hBD\u20103 peptide was more frequently detected in the basal layer as compared to the patients (53% vs. 18%, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.05). In patients, hBD\u20103 expression extended from the basal layer to the spinous layers (82%), in which hBD\u20103 was confined to the basal and deep spinous layers in clinically healthy tissues from patients, whereas it extended to the superficial spinous layers in pocket tissues from patients (0% vs. 50%, <jats:italic>p<\/jats:italic>\u2003&lt;\u20030.05). In both groups, hBD\u20103 peptide was expressed not only in gingival keratinocytes, but also in Langerhans cells and Merkel cells. hBD\u20103 transcripts were detected in 90% of the samples and they were confined to the basal and\/or suprabasal layers of gingival epithelia.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> This study shows that hBD\u20103 is frequently expressed in gingival epithelia. The appropriate expression of hBD\u20103 peptide may contribute to the maintenance of periodontal homeostasis, possibly through its antimicrobial effect and promotion of adaptive immune responses.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00827.x","type":"journal-article","created":{"date-parts":[[2005,9,13]],"date-time":"2005-09-13T13:55:51Z","timestamp":1126619751000},"page":"474-481","source":"Crossref","is-referenced-by-count":72,"title":["Expression of human \u03b2\u2010defensin\u20103 in gingival epithelia"],"prefix":"10.1111","volume":"40","author":[{"given":"Qian","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshman P.","family":"Samaranayake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P.","family":"Darveau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,9,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(01)00442-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/43088"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00190.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00199.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)00076-F"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.3.568-571.1987"},{"key":"e_1_2_6_8_2","first-page":"1247","article-title":"Gene expression, immunolocalization, and secretion of human defensin\u20105 in human female reproductive tract","volume":"152","author":"Quayle AJ","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81160-2"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.6.2389-2395.1997"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008557200"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0865fje"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00687-5"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00727.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.6.2740-2745.1999"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)01123-4"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-2-20"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021481"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2002.11186.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004410100433"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.6.3262"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260807.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00200527"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(00)00050-9"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2001.360503.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.525"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.01374.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.06.006"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2005.00827.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2005.00827.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T01:18:22Z","timestamp":1697332702000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2005.00827.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,13]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["10.1111\/j.1600-0765.2005.00827.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2005.00827.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,13]]}}}