{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:39:14Z","timestamp":1791142754396,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,31]],"date-time":"2006-01-31T00:00:00Z","timestamp":1138665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p><jats:bold>Background and objective:\u2002<\/jats:bold> Calprotectin is composed of two proteins, S100A8 and S100A9, which are S100 family members, and is detected in gingival crevicular fluid and gingival tissue with inflammation. The release and production of calprotectin are regulated by lipopolysaccharides of periodontopathic bacteria and cytokines. Emotional or psychological stress, a risk factor of periodontal disease, is transmitted by stress modulators including norepinephrine and cortisol. The aim of the present study was to investigate the effect of stress on calprotectin expression using norepinephrine and cortisol.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> U\u2010937 cells, a human monocytic cell line, were incubated with norepinephrine in the presence or absence of \u03b2\u2010 or \u03b1\u2010adrenergic receptor antagonists, or with cortisol. The expression of S100A8\/S100A9 mRNAs was examined by northern blotting and the amount of calprotectin was measured by enzyme\u2010linked immunosorbent assay (ELISA). The DNA binding activity of C\/EBP\u03b1 (CCAAT enhancing binding protein), a transcription factor, was examined by electrophoretic mobility shift assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Norepinephrine stimulated the expression of S100A8\/S100A9 mRNAs via \u03b2\u2010adrenergic receptors in U\u2010937 cells and significantly increased calprotectin production to about 3.6\u2010fold that of the control. However, cortisol had no effect on calprotectin expression at the mRNA and protein levels. Norepinephrine elevated C\/EBP\u03b1 DNA binding activity, but cortisol did not increase the activity.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Norepinephrine, a stress modulator, stimulated calprotectin expression in human monocytic cells. Calprotectin expression may be regulated by stress in addition to inflammatory factors.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2005.00845.x","type":"journal-article","created":{"date-parts":[[2006,1,31]],"date-time":"2006-01-31T11:14:02Z","timestamp":1138706042000},"page":"159-164","source":"Crossref","is-referenced-by-count":16,"title":["Norepinephrine stimulates calprotectin expression in human monocytic cells"],"prefix":"10.1111","volume":"41","author":[{"family":"Suryono","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010ichi","family":"Kido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatoshi","family":"Kataoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,31]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"187","volume-title":"Stimulus response coupling: the role of intracellular calcium\u2010binding proteins","author":"Fagerhol MK","year":"1990"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(98)00144-X"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365518409083820"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1995.tb00159.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.12.1719"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1998.tb02340.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.261004.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00691.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0902-0055.2004.00139.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.3.437"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)93237-J"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4242-4247.2001"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10299"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1996.tb00087.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.288"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.7.786"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1255"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(98)80070-3"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.12.761"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1159\/000288679"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(00)00205-8"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-1591(02)00036-3"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.275.3.R870"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2002.170506.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(89)90414-7"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205521"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(11)80541-3"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.44.28545"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(98)80070-3"},{"key":"e_1_2_6_31_2","first-page":"S36","article-title":"Signal transduction in brown adipose tissue recruitment: noradrenaline and beyond","volume":"20","author":"Cannon B","year":"1996","journal-title":"Int J Obes Relat Metab Disord"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2767"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2005.00845.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2005.00845.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T19:34:04Z","timestamp":1697052844000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2005.00845.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,31]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1111\/j.1600-0765.2005.00845.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2005.00845.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,31]]}}}