{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:38:13Z","timestamp":1773322693330,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T00:00:00Z","timestamp":1148947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> It is known that epithelial islands are embedded in the cementum during tooth root formation, but details of this process remain unknown. The purpose of this study was to investigate the dynamic characteristics of Malassez's epithelial rest cells in the cementum during tooth root formation in pigs <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> The first molars of 6\u2010mo\u2010old pigs were used in this study. Specimens were decalcified before being embedded in paraffin. Paraffin sections were investigated using TdT\u2010mediated dUTP\u2010biotin nick end labeling (TUNEL), immunohistochemical, and ultrastructural techniques.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Malassez's epithelial rest cells were located close to the root surface at the apical one\u2010third of the periodontal ligament, and epithelial clusters surrounded by distinct lamina cementia were sometimes observed in the cementum. TUNEL\u2010positive cells were detected only in the cementum. Malassez's epithelial rest cells in the periodontal ligament were completely surrounded by basement membranes, but epithelial clusters in the cementum were only intermittently surrounded by such membranes. Cytokeratin\u2010positive cells in the superstratum of the cementum were directly connected by cementocytes and by desmosome\u2010like structures. However, organelles were scarce in the cytokeratin\u2010positive cells in the substratum of the cementum, and the matrix of the cementum was deposited in the cells.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results suggest that the majority of the fragmented Hertwig's root sheath remains in the periodontal ligament and that some cells, which are connected to cementoblasts, are embedded in the cementum and progress to apoptosis.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2006.00871.x","type":"journal-article","created":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T18:27:50Z","timestamp":1149013670000},"page":"280-287","source":"Crossref","is-referenced-by-count":18,"title":["Morphology of Malassez's epithelial rest\u2010like cells in the cementum: transmission electron microscopy, immunohistochemical, and TdT\u2010mediated dUTP\u2010biotin nick end labeling studies"],"prefix":"10.1111","volume":"41","author":[{"given":"M.","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Matsuzaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Shimono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00854.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90020-3"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2330\/joralbiosci1965.39.618"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90005-8"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1980.51.8.437"},{"key":"e_1_2_6_7_2","first-page":"90","article-title":"An ultrastructural evaluation of the relationship between epithelial rests of Malassez and orthodontic root resorption and repair in man","volume":"12","author":"Brice GL","year":"1991","journal-title":"Aust Orthod J"},{"key":"e_1_2_6_8_2","first-page":"153","article-title":"Periodontal neural endings intimately relate to epithelial rests of Malassez in humans. A light and electron microscope study","volume":"182","author":"Lambrichts I","year":"1993","journal-title":"J Anat"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(62)90044-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750090701"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016357009028248"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(90)90416-P"},{"key":"e_1_2_6_13_2","first-page":"129","article-title":"Sur l'existance d'amas epitheliaux autour de la roune des dents chez l'homme adulte et a l'etat nomal","volume":"5","author":"Malassez L","year":"1885","journal-title":"Arch Physiol Norm Pathol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1993.tb00324.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1969.40.2.87"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.52.61"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2006.00871.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2006.00871.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T19:37:23Z","timestamp":1697053043000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2006.00871.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6,26]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1600-0765.2006.00871.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2006.00871.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6,26]]}}}