{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:54:34Z","timestamp":1791888874057,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,5,17]],"date-time":"2006-05-17T00:00:00Z","timestamp":1147824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Recent findings have suggested that osteoclastogenesis is directly regulated by receptor activator of nuclear factor\u2010kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). However, no studies have described interactions of OPG\/RANKL and the gp130 cytokine family in periodontal disease. This study aimed to identify and quantify OPG\/RANKL in the gingival crevicular fluid (GCF) and connective tissue of patients with periodontitis, and to clarify possible correlations with disease severity and interleukin\u20106 (IL\u20106) cytokines.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Ninety\u2010five sites in 20 patients with generalized chronic periodontitis were divided into four groups by site based on probing depth (PD) and bleeding on probing (BOP). In periodontitis patients, GCF was obtained using sterile paper strips from clinically healthy sites (PD\u2003\u2264\u20033\u2003mm without BOP, <jats:italic>n<\/jats:italic>\u2003=\u200312 in periodontitis subjects), mildly diseased sites (PD\u2003\u2264\u20033\u2003mm with BOP, <jats:italic>n<\/jats:italic>\u2003=\u200323), moderately diseased sites (PD\u2003\u2264\u20034\u20136\u2003mm with BOP, <jats:italic>n<\/jats:italic>\u2003=\u200333) and severely diseased sites (PD\u2003&gt;\u20036\u2003mm with BOP, <jats:italic>n<\/jats:italic>\u2003=\u200327). Fourteen clinically healthy sites from four periodontally healthy individuals were used as the control group. The levels of OPG, RANKL and two\u2003gp130 cytokines \u2013 IL\u20106 and oncostatin M (OSM) \u2013 in the GCF were determined by an enzyme\u2010linked immunosorbent assay (ELISA) and are expressed as total amounts (pg\/site). Immunohistochemical localization of OPG\u2010 and RANKL\u2010positive cells was also performed on gingival connective tissues harvested from patients with periodontitis (inflammatory group, <jats:italic>n<\/jats:italic>\u2003=\u20038 biopsies) and from non\u2010diseased individuals (healthy group, <jats:italic>n<\/jats:italic>\u2003=\u20038 biopsies).<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> GCF RANKL, but not OPG, was elevated in diseased sites of patients with periodontitis. However, the expressions of OPG and RANKL showed no correlation with disease severity (<jats:italic>r\u2003=<\/jats:italic>\u20030.174 and 0.056, respectively), but the content of RANKL in the GCF was significantly positively correlated with those of IL\u20106 (<jats:italic>r\u2003=<\/jats:italic>\u20030.207) and OSM (<jats:italic>r\u2003=<\/jats:italic>\u20030.231) (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.01). Immunohistochemical staining showed that RANKL\u2010positive cells were significantly distributed in the inflammatory connective tissue zone of diseased gingiva, compared with those of samples from non\u2010diseased persons (<jats:italic>p\u2003&lt;<\/jats:italic>\u20030.01). However, few OPG\u2010positive cells were found in connective tissue zones of either the diseased gingiva or healthy biopsies.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These findings imply that in this cross\u2010sectional study of GCF, RANKL, IL\u20106 and OSM were all prominent in periodontitis sites, whereas OPG was inconsistently found in a few samples of diseased sites but was undetectable in any of the control sites. The results also imply that the expression of RANKL was positively correlated with IL\u20106 and OSM in the GCF.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2006.00883.x","type":"journal-article","created":{"date-parts":[[2006,5,17]],"date-time":"2006-05-17T09:19:32Z","timestamp":1147857572000},"page":"354-360","source":"Crossref","is-referenced-by-count":100,"title":["Identification of the osteoprotegerin\/receptor activator of nuclear factor\u2010kappa B ligand system in gingival crevicular fluid and tissue of patients with chronic periodontitis"],"prefix":"10.1111","volume":"41","author":[{"given":"H.\u2010K.","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.\u2010L.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010C.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.\u2010L.","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Y.\u2010P.","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,6,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.12.8536"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/36593"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3540"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3597"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80209-3"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.3.5837"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10763"},{"key":"e_1_2_6_9_2","first-page":"17","article-title":"Expression of RANKL and OPG mRNA in periodontal disease: possible involvement in bone destruction","volume":"11","author":"Liu D","year":"2003","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300216"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119508"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200002)43:2<281::AID-ANR7>3.0.CO;2-7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.4049\/jimmunol.147.7.2175","article-title":"Regulation of IL\u20106 expression by oncostatin M","volume":"147","author":"Brown TJ","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.12.018"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00737.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750010801"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1999.0635"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.4.8625883"},{"key":"e_1_2_6_19_2","first-page":"559","article-title":"Interleukin\u20106\u2010type cytokines stimulates mesenchymal progenitor differentiation toward the osteoblastic lineage","volume":"110","author":"Taguchi Y","year":"1998","journal-title":"Proc Assoc Am Physicians"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(95)00482-3"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1461"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118432"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00815.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/16852"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(00)01718-7"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3379"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.5.997"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00615.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/hq1201.100229"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.2.631"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.9.1260"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2006.00883.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2006.00883.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T19:38:36Z","timestamp":1697053116000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2006.00883.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6,26]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1600-0765.2006.00883.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2006.00883.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6,26]]}}}