{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:28:19Z","timestamp":1780486099340,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> The purpose of this study was to investigate the effects of chemically modified tetracyclines (CMTs) on the production of gelatinases [matrix metalloproteinase (MMP)\u20102 and \u20109] by human periodontal ligament (PDL) cells, and on the activity of recombinant gelatinases.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Human PDL cells were cultured with CMT\u20101, \u20103, \u20105, \u20107 or \u20108 in concentrations of 0, 1, 5, 10, 20, 50, 100, 200 and 500\u2003\u00b5<jats:sc>m<\/jats:sc>. Gelatin zymography was used to determine MMP\u20102 and \u20109 production of the cells. The amount of DNA present in the cultures was analyzed using a fluorescent assay. The cytotoxicity of the CMTs was also determined. Recombinant human MMP\u20102 and \u20109 were incubated with the CMTs (0\u2013500\u2003\u00b5<jats:sc>m<\/jats:sc>) and their activity was analyzed using an internally quenched fluorogenic substrate.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> MMP\u20102 production was stimulated up to sevenfold by CMT\u20101, \u20103, \u20107 and \u20108 at low concentrations (10\u2013200\u2003\u00b5<jats:sc>m<\/jats:sc>). No significant amounts of MMP\u20109 were produced. In contrast, MMP\u20102 and \u20109 activity was reduced by \u2248\u200a10\u201340\u2010fold at higher concentrations (200\u2013500\u2003\u00b5<jats:sc>m<\/jats:sc>). CMT\u20105 had no effect on the production or on the activity of MMP\u20102 and \u20109. Only CMT\u20103 and \u20108 had cytotoxic effects on the PDL cells at the highest concentrations.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Surprisingly, CMTs are able to stimulate MMP\u20102 production at relatively low concentrations. However, at higher concentrations they exert a much stronger inhibitory effect on gelatinase activity. A possible stimulatory effect of CMTs on MMP production should be considered in their clinical use.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2006.00893.x","type":"journal-article","created":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T15:01:46Z","timestamp":1153148506000},"page":"463-470","source":"Crossref","is-referenced-by-count":6,"title":["Chemically modified tetracyclines stimulate matrix metalloproteinase\u20102 production by periodontal ligament cells"],"prefix":"10.1111","volume":"41","author":[{"given":"M. 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