{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T23:30:15Z","timestamp":1699918215819},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,11,27]],"date-time":"2006-11-27T00:00:00Z","timestamp":1164585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Platelet\u2010activating factor is elevated in localized aggressive periodontitis. We previously demonstrated that the elevated level of platelet\u2010activating factor in localized aggressive periodontitis is at least partially attributable to low levels of platelet\u2010activating factor acetylhydrolase, the enzyme that catabolizes platelet\u2010activating factor. The objective of this study was to determine if platelet\u2010activating factor synthesis was also elevated in localized aggressive periodontitis. To test this, platelet\u2010activating factor synthesis was quantified in the monocytes and polymorphonuclear neutrophils of periodontally healthy patients and of subjects with localized aggressive periodontitis.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Cells were labeled with [<jats:sup>3<\/jats:sup>H]acetate and treated with vehicle or stimulated with calcium ionophore A23187. Platelet\u2010activating factor was extracted and quantified by scintillation counting.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> For both subject groups, resting monocytes and polymorphonuclear neutrophils produced platelet\u2010activating factor, and calcium ionophore A23187 stimulated platelet\u2010activating factor production in both cell types. However, calcium ionophore A23187\u2010activated monocytes from subjects with localized aggressive periodontitis produced less platelet\u2010activating factor than did activated periodontally healthy monocytes (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.0001), suggesting an aberrant calcium ionophore A23187 response in monocytes from subjects with localized aggressive periodontitis. Indeed, when the data were expressed as fold induction of platelet\u2010activating factor synthesis in response to calcium ionophore A23187, monocytes from subjects with localized aggressive periodontitis exhibited only a fourfold increase in platelet\u2010activating factor synthesis, whereas calcium ionophore A23187\u2010stimulated monocytes from periodontally healthy, chronic periodontitis and generalized aggressive periodontitis subjects produced \u2248\u200a12 times more platelet\u2010activating factor than did resting monocytes. In contrast, both resting and activated localized aggressive periodontitis polymorphonuclear neutrophils synthesized more platelet\u2010activating factor than did periodontally healthy polymorphonuclear neutrophils.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These data suggest that high levels of platelet\u2010activating factor in subjects with localized aggressive periodontitis result from both increased synthesis and reduced catabolism. While localized aggressive periodontitis polymorphonuclear neutrophils contribute to increased platelet\u2010activating factor mass through synthesis, the contribution of monocytes is probably the result of reduced catabolism by platelet\u2010activating factor acetylhydrolase.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2006.00933.x","type":"journal-article","created":{"date-parts":[[2006,11,27]],"date-time":"2006-11-27T10:04:08Z","timestamp":1164621848000},"page":"202-211","source":"Crossref","is-referenced-by-count":3,"title":["Differential platelet\u2010activating factor synthesis by monocytes and polymorphonuclear leukocytes from subjects with localized aggressive periodontitis"],"prefix":"10.1111","volume":"42","author":[{"given":"C. R.","family":"Shin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Moores","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Best","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G.","family":"Tew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A.","family":"Schenkein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. E.","family":"Barbour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,19]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"491","article-title":"A review of localized juvenile periodontitis (LJP): Part I. Clinical features, epidemiology, etiology, and pathogenesis","volume":"46","author":"Gustke CJ","year":"1998","journal-title":"Gen Dent"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1974.45.8.2.636"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.7.730"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.3s.282"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1128\/iai.64.9.3908-3910.1996","article-title":"Influence of anti\u2010Actinobacillus actinomycetemcomitans Y4 (serotype b) lipopolysaccharide on severity of generalized early\u2010onset periodontitis","volume":"64","author":"Califano JV","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1128\/iai.62.5.1677-1682.1994","article-title":"Serum immunoglobulin G subclass concentrations in peridontally healthy and diseased individuals","volume":"62","author":"Lu H","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1987.58.5.314"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1128\/iai.55.9.2262-2267.1987","article-title":"Association of an abnormality of neutrophil chemotaxis in human periodontal disease with a cell surface protein","volume":"55","author":"Van Dyke TE","year":"1987","journal-title":"Infect Immun"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1986.57.11.703"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0105023"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2004-2009.1996"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.2780-2786.2002"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.1995.tb00894.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.259.5.C697"},{"key":"e_1_2_6_16_2","first-page":"21652","article-title":"Phospholipid remodeling in human neutrophils. Parallel activation of a deacylation\/reacylation cycle and platelet\u2010activating factor synthesis","volume":"264","author":"Reinhold SL","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003164"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740040401"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110020701"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90044-7"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.8.1032"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1995.66.7.613"},{"key":"e_1_2_6_23_2","first-page":"720","article-title":"Delineation of the role of platelet\u2010activating factor in the immunoglobulin G2 antibody response","volume":"11","author":"Al Darmaki S","year":"2004","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1563-1568.2000"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.1.167"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.2000.1648"},{"key":"e_1_2_6_27_2","doi-asserted-by":"crossref","first-page":"3918","DOI":"10.4049\/jimmunol.138.11.3918","article-title":"Production of platelet\u2010activating factor by stimulated human polymorphonuclear leukocytes. Correlation of synthesis with release, functional events, and leukotriene B4 metabolism","volume":"138","author":"Sisson JH","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(97)00022-2"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1202601"},{"key":"e_1_2_6_31_2","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.4049\/jimmunol.140.5.1618","article-title":"Synthesis and release of platelet\u2010activating factor by stimulated human mononuclear phagocytes","volume":"140","author":"Elstad MR","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_6_32_2","doi-asserted-by":"crossref","first-page":"9682","DOI":"10.1016\/S0021-9258(19)38724-1","article-title":"Human macrophages secrete platelet\u2010activating factor acetylhydrolase","volume":"265","author":"Stafforini DM","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.11.6474"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.5.797"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.7.617"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.8.3137-3142.1993"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00870.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.2.139"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.3.224"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1995.66.5.321"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2500-2505.1996"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1856"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00680.x"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.6.2481-2487.1992"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.419"},{"key":"e_1_2_6_46_2","doi-asserted-by":"crossref","first-page":"15370","DOI":"10.1016\/S0021-9258(18)47734-4","article-title":"Protein kinase C activity appears to be required for the synthesis of platelet\u2010activating factor and leukotriene B4 by human neutrophils","volume":"262","author":"McIntyre TM","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_6_47_2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.4049\/jimmunol.151.1.339","article-title":"Influence of isoproterenol and phosphodiesterase inhibitors on platelet\u2010activating factor biosynthesis in the human neutrophil","volume":"151","author":"Fonteh AN","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2680091"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408535102"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(02)00314-2"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.24.6973"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.1.66"},{"key":"e_1_2_6_53_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1096\/fj.05-4724fje","article-title":"RvE1 protects from local inflammation and osteoclast\u2010mediated bone destruction in periodontitis","volume":"20","author":"Hasturk H","year":"2005","journal-title":"FASEB J"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1986.tb01443.x"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03110.x"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5s.432"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi992551b"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1810-9_5"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2006.00933.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2006.00933.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T19:00:10Z","timestamp":1696964410000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2006.00933.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,19]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1600-0765.2006.00933.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2006.00933.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,19]]}}}