{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:34:09Z","timestamp":1790469249574,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,3]],"date-time":"2007-05-03T00:00:00Z","timestamp":1178150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> We have previously shown that increases in neutrophil elastase in periodontal ligament with chronic periodontitis results in degradation of the noncollagenous components. The purpose of this study was to investigate whether the destruction of noncollagenous components by treatment with elastase <jats:italic>in vitro<\/jats:italic> causes changes in the mechanical properties of the periodontal ligament.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> The transverse sections of mandibular first molars, prepared from male Wistar rats at 6\u2003wk of age, were digested with 0\u201350\u2003\u00b5g\/mL of neutrophil elastase at 37\u00b0C for 4\u2003h. Then, their mechanical properties and morphological features were examined.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Digestion with elastase dose\u2010dependently decreased the maximum shear stress and failure strain energy density of the periodontal ligament (<jats:italic>p<\/jats:italic>\u2003&lt;\u20030.05\u20130.01). The histological observations after digestion revealed marked degradation of oxytalan fibers, but no marked changes of the collagen fibers, which was confirmed by the detection of very low quantities of hydroxyproline in the digest. The light and scanning electron micrographs showed that the elastase degraded the interfibrillar substances in the periodontal ligament and exposed individual collagen fibrils.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results suggest that the increased neutrophil elastase observed in periodontal disease degrades the oxytalan fibers and interfibrillar substances in the periodontal ligament to decrease its mechanical strength.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2007.00990.x","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T04:36:47Z","timestamp":1178685407000},"page":"22-31","source":"Crossref","is-referenced-by-count":17,"title":["Degradation of noncollagenous components by neutrophil elastase reduces the mechanical strength of rat periodontal ligament"],"prefix":"10.1111","volume":"43","author":[{"given":"Y.","family":"Ujiie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Komatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Gomi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Oida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fukae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345770560080501"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1992.tb01674.x"},{"key":"e_1_2_6_4_2","first-page":"215","volume-title":"The periodontal ligament in health and disease","author":"Moxham BJ","year":"1995"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.2330\/joralbiosci1965.42.193"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5s.474"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03106.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2004.01038.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1997.68.6.517"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028002128.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00663.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2002.290308.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.188"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00705.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.1.66"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.7.702"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1995.tb00121.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1995.tb01798.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00532.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1996.tb00501.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00129.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.2329\/perio.45.22"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00952.x"},{"key":"e_1_2_6_24_2","first-page":"231","article-title":"Identification of proteinase by zymo\u2010Western analysis using zymography combined Western analysis \u2013 identification of MMP\u20109 in periodontal ligament on human chronic periodontitis","volume":"30","author":"Ujiie Y","year":"2004","journal-title":"Tsurumi Univ Dent J"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1254\/fpj.122.151"},{"key":"e_1_2_6_26_2","first-page":"3347","article-title":"Studies on the in vitro, ex vivo and in vivo pharmacological profile of a neutrophil elastase inhibitor, ONO\u20105046\u00b7Na [in Japanese]","volume":"31","author":"Nakao S","year":"1997","journal-title":"Kiso To Rinsho"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(93)90207-3"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209709160212"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(93)90017-9"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(88)90102-1"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(86)90169-7"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(86)90190-9"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1974.tb01724.x"},{"key":"e_1_2_6_34_2","first-page":"251","volume-title":"The connective tissue","author":"Spicer SS","year":"1967"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(95)00114-X"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209609028886"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)80046-8"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(85)90043-3"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345920710121001"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a123277"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00921.2004"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.10.001"},{"key":"e_1_2_6_43_2","first-page":"1","article-title":"In vitro effects of ehtylenediaminetetraacetic acid and pH on biomechanical properties and morphologic features of the rat molar periodontal ligament","volume":"5","author":"Kanazashi M","year":"1999","journal-title":"J\u00a0Oromax Biomech"},{"key":"e_1_2_6_44_2","first-page":"131","volume-title":"Biochemistry: solubilities of proteins","author":"Voet D","year":"2004"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300897"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.281.4.L868"},{"key":"e_1_2_6_47_2","first-page":"3","article-title":"Lytic effect of human leukocytic proteases on gingival tissue [in French]","volume":"8","author":"Heiniger R","year":"1980","journal-title":"J\u00a0Biologie Buccale"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1982.tb01142.x"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1995.tb01253.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2007.00990.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2007.00990.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T01:34:12Z","timestamp":1696988052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2007.00990.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,25]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1111\/j.1600-0765.2007.00990.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2007.00990.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,25]]}}}