{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:08:00Z","timestamp":1790791680655,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,7,4]],"date-time":"2008-07-04T00:00:00Z","timestamp":1215129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Conventional selective media have been used for the selection of <jats:italic>Aggregatibacter<\/jats:italic> (<jats:italic>Actinobacillus<\/jats:italic>) <jats:italic>actinomycetemcomitans<\/jats:italic> in clinical samples. The proportion of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> grown on the selective media <jats:italic>in vitro<\/jats:italic> may not reflect the true counts <jats:italic>in vivo<\/jats:italic> because of the low selectivity. A novel selective medium, designated AASM, was developed for the isolation of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> AASM was prepared by adding of 200\u2003\u03bcg\/mL of vancomycin and 10\u2003U\/mL of bacitracin to AAGM, which contains dextrose, sodium bicarbonate, trypticase soy, yeast extract and agar. Clinical efficacy was evaluated by the recovery, on AASM, of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> from subgingival samples of 44 periodontally healthy subjects and 76 patients with chronic periodontitis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All serotypes (a\u2013f) of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> strains grew well, and the average growth recovery of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> on AASM medium was 94.4% (80.0\u2013109.7%) of that on AAGM. The exclusive rate of other bacteria was 99.9% in clinical samples cultured on AASM. <jats:italic>A. actinomycetemcomitans<\/jats:italic> was not detected in periodontally healthy persons but was detected in 25 (32.9%) patients with chronic periodontitis. The predominant serotype was c, detected in 11 subjects.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The new selective medium, AASM, was highly selective for <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic>, eliminated possible false\u2010positive results and was useful for the isolation of <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> from clinical samples.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2007.01074.x","type":"journal-article","created":{"date-parts":[[2008,7,6]],"date-time":"2008-07-06T01:25:48Z","timestamp":1215307548000},"page":"544-548","source":"Crossref","is-referenced-by-count":20,"title":["A novel selective medium for isolation of <i>Aggregatibacter<\/i> (<i>Actinobacillus<\/i>) 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