{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:14:52Z","timestamp":1767665692299},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,7]],"date-time":"2009-01-07T00:00:00Z","timestamp":1231286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> The role of human leukocyte histocompatibility antigen (HLA) class\u2003II molecules on non\u2010antigen\u2010presenting cells has been a matter of controversy. We previously reported that HLA\u2010II molecules on human gingival fibroblasts (GF) do not present antigens, but transduce signals into the cells, resulting in the expression of several cytokines, such as interleukin\u20106 (IL\u20106), monocyte chemoattractant protein\u20101 (MCP\u20101), regulated upon activation, normal T\u2010cell expressed and secreted (RANTES) and IL\u20108. However, the exact role of these cytokines, as well as other cytokines which are potentially secreted from GF, in the pathogenesis of chronic periodontal inflammation is not fully understood. The aim of this study was to observe the effects of HLA\u2010II\u2010induced cytokines on the proliferation of human umbilical vein endothelial cells (HUVEC).<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Antibody\u2010based cytokine\u2010microarray analyses were performed to detect potential cytokines associated with angiogenesis. Next, cytokine productivity was confirmed by quantitative methods. Then, cell proliferation assay was performed to see whether these cytokines promoted the proliferation of HUVEC.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Besides IL\u20106, MCP\u20101, RANTES and IL\u20108, growth\u2010related gene product (GRO) was newly identified as an HLA\u2010II\u2010induced cytokine released from GF. This was confirmed by a quantitative method. Cell culture supernatant from HLA\u2010II\u2010stimulated GF cultures promoted the growth of HUVEC. Addition of anti\u2010IL\u20108 neutralizing antibody, anti\u2010CXC receptor (CXCR)1 antibody and anti\u2010MCP\u20101 antibody inhibited the growth of HUVEC in a dose\u2010dependent manner, while addition of anti\u2010GRO\u03b1 antibody did not.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The HLA\u2010II\u2010induced IL\u20108, via CXCR1, as well as MCP\u20101 from GF, promotes endothelial cell proliferation, which is possibly associated with enhanced angiogenesis in chronic periodontal lesions.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01097.x","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T06:15:45Z","timestamp":1231395345000},"page":"103-109","source":"Crossref","is-referenced-by-count":14,"title":["Human leukocyte histocompatibility antigen class II\u2010induced cytokines from human gingival fibroblasts promote proliferation of human umbilical vein endothelial cells: potential association with enhanced angiogenesis in 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