{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:37:16Z","timestamp":1760888236263},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,7,4]],"date-time":"2008-07-04T00:00:00Z","timestamp":1215129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are known to be involved in the periodontal disease process. Results of in vivo MMPs and TIMPs gene expressions in the gingiva, though, are still controversial. In the present study, we compared the gene expression of MMP\u20101, \u20102, \u20109, \u201013 and TIMP\u20101, \u20102 in healthy and inflamed gingiva.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> 38 gingival samples were collected from gingivitis (n\u2003=\u200310), advanced chronic periodontitis (n\u2003=\u200310), generalized aggressive periodontitis (n\u2003=\u20038) and periodontally healthy individuals (n\u2003=\u200310). Total RNA isolated from those samples was subjected to reverse transcription followed by amplification by polymerase chain reaction (RT\u2010PCR). Products were visualized in agarose gels and quantified by optical densitometry. Samples were also processed for gelatin zymography and Western blotting for MMP\u20102 and MMP\u20109 in order to assess for post\u2010transcriptional MMP regulation at the protein level.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The frequencies and levels of transcripts encoding MMPs and TIMPs were found to be not significantly different among groups (p\u2003&gt;\u20030.05, Fisher\u2019s Exact and Kruskall\u2010Wallis tests). There is a trend towards higher MMP\u20102 and \u20109 gelatinase activities in the inflamed samples, although not statistically significant. In contrast, zymography and Western blotting studies show that MMP\u20102 is virtually absent in the chronic periodontitis group.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results could reflect a complex regulation of MMPs and TIMPs\u2019 gene expression in the course of gingival inflammation. They also reveal a great biological diversity even among individuals with similar periodontal status.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01101.x","type":"journal-article","created":{"date-parts":[[2008,7,6]],"date-time":"2008-07-06T01:28:14Z","timestamp":1215307694000},"page":"570-577","source":"Crossref","is-referenced-by-count":23,"title":["Expression of metalloproteinases and their tissue inhibitors in inflamed gingival biopsies"],"prefix":"10.1111","volume":"43","author":[{"given":"L. D. R.","family":"Gon\u00e7alves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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J.","family":"Feres\u2010Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/07853890600800103"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00191.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.1996.tb00142.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790120801"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028002128.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.196"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00705.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00545.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00786.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.060144"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050288"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050293"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050461"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01244.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00888.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2006.05.014"},{"key":"e_1_2_6_18_2","first-page":"247","article-title":"Levels of metalloproteinase\u20102 and \u20109 and tissue inhibitor of matrix metalloproteinase\u20101 in gingival crevicular fluid of patients with periodontitis, gingivitis, and healthy gingiva","volume":"38","author":"Maeso G","year":"2007","journal-title":"Quintessence Int"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(79)90112-2"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00696.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.10.1387"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00532.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00816.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1996.tb01814.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(95)00126-3"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1988.tb01004.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.6.974"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb00761.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750121101"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2007.00993.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2005.03.010"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2005.01.004"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(03)00007-8"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2008.01101.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2008.01101.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T01:18:33Z","timestamp":1697332713000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2008.01101.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,26]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1111\/j.1600-0765.2008.01101.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2008.01101.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,26]]}}}