{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:29:38Z","timestamp":1791217778232,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,6]],"date-time":"2009-02-06T00:00:00Z","timestamp":1233878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> This study analyzed the interaction of <jats:italic>Porphyromonas gingivalis<\/jats:italic> ATCC 33277 and <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to <jats:italic>P.\u2003gingivalis<\/jats:italic> and <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> following opsonization of the bacteria using the patient\u2019s own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol\u2010dependent and isoluminol\u2010dependent chemiluminescence.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Polymorphonuclear neutrophils from patients with chronic (62.16\u2003\u00b1\u200319.39%) and aggressive (43.26\u2003\u00b1\u200326.63%) periodontitis phagocytosed more <jats:italic>P.\u2003gingivalis<\/jats:italic> than the healthy controls (24.43\u2003\u00b1\u200319.87%) at the 30\u2010min time point after exposure to the bacteria (<jats:italic>p\u2003<\/jats:italic>&lt;\u20030.05). High serum IgG levels against <jats:italic>P.\u2003gingivalis<\/jats:italic> and <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> were detected in subjects with periodontitis. Polymorphonuclear neutrophils from subjects with chronic and aggressive periodontitis released significantly more reactive oxygen species and demonstrated greater human neutrophil elastase activity in the absence of any stimulus than polymorphonuclear neutrophils from healthy controls (<jats:italic>p\u2003<\/jats:italic>&lt;\u20030.05). Polymorphonuclear neutrophils in chronic periodontitis released significantly more reactive oxygen species when exposed to <jats:italic>P.\u2003gingivalis<\/jats:italic> and <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> than polymorphonuclear neutrophils in aggressive periodontitis.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> High serum IgG levels against <jats:italic>P.\u2003gingivalis<\/jats:italic> and <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> promote phagocytosis in periodontitis. The extracellular release of reactive oxygen species and neutrophil elastase by polymorphonuclear neutrophils may also contribute to damage of the surrounding periodontal tissues.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01113.x","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T06:55:48Z","timestamp":1233989748000},"page":"368-377","source":"Crossref","is-referenced-by-count":64,"title":["Neutrophils in chronic and aggressive periodontitis in interaction with <i>Porphyromonas gingivalis<\/i> and <i>Aggregatibacter actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"44","author":[{"given":"A.","family":"Guentsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Puklo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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