{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:20:42Z","timestamp":1761675642102},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T00:00:00Z","timestamp":1236902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Live\u2010animal micro\u2010computed tomography is a new and promising technique that can be used to quantify changes in bone volume for periodontal disease models. The major aim of this study was to develop the methodology of live\u2010animal micro\u2010computed tomography and to determine the effect of a novel secretory phospholipase A<jats:sub>2<\/jats:sub> inhibitor on alveolar bone loss.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Periodontitis was induced in mice by oral infection with <jats:italic>Porphyromonas gingivalis<\/jats:italic> over a period of 13\u2003wk, and live\u2010animal micro\u2010computed tomography scans were taken at different time\u2010points to determine bone volume changes with disease progression. This enabled conclusions to be made as to when treatment was most likely to be effective. In addition, the model was used to investigate a novel drug, the secretory phospholipase A<jats:sub>2<\/jats:sub> inhibitor, KHO64, and its potential ability to inhibit osteoclast bone resorption and treat periodontitis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The results from live\u2010animal micro\u2010computed tomography scans revealed greater, statistically significant, bone volume loss in diseased mice compared with normal mice (<jats:italic>p\u2003<\/jats:italic>&lt;\u20030.05). This corresponded to a larger area from the cemento\u2013enamel junction to the alveolar bone crest, as assessed by stereo imaging (<jats:italic>p\u2003<\/jats:italic>&lt;\u20030.001). These techniques can therefore detect and quantify alveolar bone loss. Both methods revealed that KHO64 had no significant effect on the volume of bone resorption.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Live\u2010animal micro\u2010computed tomography is a robust, reproducible technique that clearly demonstrates significant time\u2010dependent changes in alveolar bone volume in a small\u2010animal model of periodontitis.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01132.x","type":"journal-article","created":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T10:08:14Z","timestamp":1236938894000},"page":"317-322","source":"Crossref","is-referenced-by-count":24,"title":["The use of live\u2010animal micro\u2010computed tomography to determine the effect of a novel phospholipase A<sub>2<\/sub> inhibitor on alveolar bone loss in an <i>in vivo<\/i> mouse model of periodontitis"],"prefix":"10.1111","volume":"44","author":[{"given":"M. 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