{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T18:47:53Z","timestamp":1783968473196,"version":"3.55.0"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T00:00:00Z","timestamp":1223337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> Little is known about the factors that affect the microbial composition of supragingival biofilms. This study was designed to examine the relationship between total DNA probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> Supragingival plaque samples were taken from 187 systemically healthy adult subjects (<jats:italic>n<\/jats:italic>\u2003=\u20034745 samples). All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA\u2013DNA hybridization. The relationship between total DNA probe counts and microbial composition was examined by subsetting the data into 10\u2003groups based on 10\u2003percentile increments of the total DNA probe counts. Differences among groups in terms of species counts and proportions were sought, as well as relationships of total plaque DNA probe count and clinical parameters.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> There was a wide distribution in mean total DNA probe counts among the 187\u2003subjects. With increasing total plaque levels there was a change in the proportions of individual species and microbial complexes. \u2018Small plaques\u2019 were characterized by high proportions of species in the yellow, orange, purple and \u2018other\u2019 complexes; plaques of moderate mass were characterized by high proportions of <jats:italic>Actinomyces<\/jats:italic> and purple complex species, while \u2018large plaques\u2019 exhibited increased proportions of green and orange complex species. Measures of gingival inflammation, pocket depth and recession were significantly positively associated with total DNA probe counts. Increased plaque numbers were related to increased pocket depth irrespective of presence or absence of gingival inflammation.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The proportions of individual species and microbial complexes in supragingival biofilms are influenced by the total numbers of organisms in the biofilm.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01154.x","type":"journal-article","created":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T07:03:17Z","timestamp":1223362997000},"page":"511-519","source":"Crossref","is-referenced-by-count":49,"title":["Factors affecting human supragingival biofilm composition. I. Plaque mass"],"prefix":"10.1111","volume":"44","author":[{"given":"A. D.","family":"Haffajee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. P.","family":"Teles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Veiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. S.","family":"Socransky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1966.37.6.495"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1996.tb00532.x"},{"key":"e_1_2_6_4_2","first-page":"788","article-title":"\u201cCheckerboard\u201d DNA\u2010DNA hybridization","volume":"17","author":"Socransky SS","year":"1994","journal-title":"Biotechniques"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.2004.00168.x"},{"key":"e_1_2_6_6_2","first-page":"89","volume-title":"Statistical Ecology. 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