{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T14:30:15Z","timestamp":1773412215486,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,10,22]],"date-time":"2008-10-22T00:00:00Z","timestamp":1224633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p><jats:bold>Background and Objective:\u2002<\/jats:bold> The elastic fiber system comprises oxytalan, elaunin and elastic fibers, differing in their relative microfibril and elastin contents. Human periodontal ligaments contain oxytalan fibers (pure microfibrils). Periodontal ligaments are continuously exposed to various functional forces, such as tooth movement and occlusal loading. We have reported that bundles of microfibrils coalesce in response to mechanical strain in cultured periodontal ligament fibroblasts, as assessed in terms of their positivity for fibrillin\u20101 (the major component of microfibrils). However, the mechanism of microfibril coalescence is unclear. We hypothesized that the fibrillin\u20101\u2010binding molecule, fibulin\u20105, contributes to oxytalan fiber formation under mechanical strain.<\/jats:p><jats:p><jats:bold>Material and Methods:\u2002<\/jats:bold> We subjected periodontal ligament fibroblasts to stretching in order to examine the effects of fibulin\u20105 on the formation of oxytalan fibers in cell\/matrix layers. We transfected periodontal ligament cells with small interference RNA for fibulin\u20105, then examined oxytalan fibers using immunofluorescence and electron microscopy.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Immunofluorescence showed that fibrillin\u20101\u2010positive microfibrils coalesced as a result of stretching, compared with cells that were not subjected to stretching. Fibulin\u20105 colocalized on fibrillin\u20101\u2010positive microfibrils. Stretching increased fibulin\u20105 gene expression and protein deposition. Immunofluorescence and immunogold electron microscopy analysis revealed that fibulin\u20105 suppression inhibited the coalescence of microfibrils under stretching conditions.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results suggest that fibulin\u20105 up\u2010regulated in response to tension strain may control the formation of microfibril bundles in periodontal ligament.<\/jats:p>","DOI":"10.1111\/j.1600-0765.2008.01170.x","type":"journal-article","created":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T08:19:27Z","timestamp":1250842767000},"page":"622-627","source":"Crossref","is-referenced-by-count":25,"title":["Stretching stimulates fibulin\u20105 expression and controls microfibril bundles in human periodontal ligament cells"],"prefix":"10.1111","volume":"44","author":[{"given":"K.","family":"Nakashima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Tsuruga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Hisanaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Sawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.14.2817"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00496583"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260510.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00662.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600410"},{"key":"e_1_2_6_7_2","article-title":"Stretching modulates oxytalan fibers in human periodontal ligament cells","author":"Tsuruga E","journal-title":"J Periodontal Res"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345730520042501"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400033"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1130"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/415171a"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/415168a"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050368"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00238-3"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl414"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601768"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/6.6.425"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200148200"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1600-0765.2008.01170.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1600-0765.2008.01170.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T13:57:07Z","timestamp":1697032627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0765.2008.01170.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,21]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1111\/j.1600-0765.2008.01170.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1600-0765.2008.01170.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,21]]}}}